ArticleScientific reports2019
Variable osteogenic performance of MC3T3-E1 subclones impacts their utility as models of osteoblast biology.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 1 synthesis or guideline pooled it.
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- Osteogenic Effects ofFoods (Basel, Switzerland) · 2026Article
- Free amino acid-rich egg yolk protein hydrolysate promotes osteogenesis of MC3T3-E1 cells association withFrontiers in nutrition · 2026Article
- A cellular automaton model of osteogenic differentiation reveals identifiability limits of endpoint assays.Frontiers in systems biology · 2026Article
- 2D Boron Nanoplatelets as a Multifunctional Additive for Osteogenic, Gram-Negative Antimicrobial and Mechanically Reinforcing Bone Repair Scaffolds.Small science · 2025Article
- Rho-kinase inhibition reduces subretinal fibrosis.Cell death discovery · 2025Article
- Magnesium Ion-Mediated Regulation of Osteogenesis and Osteoclastogenesis in 2D Culture and 3D Collagen/Nano-Hydroxyapatite Scaffolds for Enhanced Bone Repair.Journal of functional biomaterials · 2025Article
- CXCR4 is a response gene for parathyroid hormone which affects osteoblast and osteoclast function in vitro.Bone & joint research · 2025Article
- Genetic Profiling of MC3T3-E1 Cells in Different Media: Implications for In Vitro Screening Development.Biomedicines · 2025Article
- Differential effects of structurally different lysophosphatidylethanolamine species on proliferation and differentiation in pre-osteoblast MC3T3-E1 cells.Scientific reports · 2025Article
- Osteogenic effects of electrophoretically deposited Sr-doped calcium silicate coatings on titanium.Frontiers in bioengineering and biotechnology · 2025Article
- Pro-differentiative, Pro-adhesive and Pro-migratory Activities of Isorhamnetin in MC3T3-E1 Osteoblasts via Activation of ERK-dependent BMP2-Smad Signaling.Cell biochemistry and biophysics · 2024Article
- Investigating the potential effect of Holothuria scabra extract on osteogenic differentiation in preosteoblast MC3T3-E1 cells.Scientific reports · 2024Article
- 3D Melt-Extrusion Printing of Medium Chain Length Polyhydroxyalkanoates and Their Application as Antibiotic-Free Antibacterial Scaffolds for Bone Regeneration.ACS biomaterials science & engineering · 2024Article
- The Mohawk homeobox gene represents a marker and osteo-inhibitory factor in calvarial suture osteoprogenitor cells.Cell death & disease · 2024Article
- Neuropilin2 in Mesenchymal Stromal Cells as a Potential Novel Therapeutic Target in Myelofibrosis.Cancers · 2024Article
- Defining the Most Potent Osteoinductive Culture Conditions for MC3T3-E1 Cells Reveals No Implication of Oxidative Stress or Energy Metabolism.International journal of molecular sciences · 2024Article
- Effect of curcumin and three analogues on pre-osteoblast cells' viability, differentiation, and gene expression.Brazilian oral research · 2024Article
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Authors and funding
2 authors.
Funding
Abstract
The spontaneously immortalized murine calvarial cell line MC3T3-E1 and its derivative subclones are widely used models of osteoblast biology. Many investigators have reported conflicting data under seemingly similar experimental conditions, though the specific subclone studied is often not specified. The purpose of this study was to directly compare the commercially available MC3T3-E1 subclones 4, 14, and 24 in terms of responsiveness to osteogenic induction media and/or stimulation with rhPTH[1-34]. We assayed osteogenic gene expression, capacity to deposit and mineralize a collagenous matrix, and the expression and signaling function of PTH1R. Our data demonstrate that each subclone bears little functional resemblance to the others, or to primary calvarial osteoblasts. Specifically, whereas subclone 4 is responsive to PTH stimulation and capable of matrix mineralization, subclones 14 and 24 do not faithfully replicate these key aspects of osteoblast biology. Furthermore, little overlap was observed between the gene expression profile of subclone 4 and primary calvarial osteoblasts. Our experience working with these cell lines demonstrates that the MC3T3-E1 derived cell lines are imperfect models of osteoblast biology, and reinforce the importance of clearly articulating selection and reporting of research materials.
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