Evidence map›Paper›PMID 31164920›Full record

ArticleJournal of biological engineering2019

Transplantation of CRISPRa system engineered IL10-overexpressing bone marrow-derived mesenchymal stem cells for the treatment of myocardial infarction in diabetic mice.

Xin Meng, Minjuan Zheng, Ming Yu, Wei Bai, Lei Zuo, Xin Bu, Yi Liu, Linying Xia, Jing Hu, Liwen Liu and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biological engineering, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
3.1field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 43 citations in OpenAlex.

  1. Review
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  3. Epicardial Adipocytes in Cardiac Pathology and Healing.Cardiovascular drugs and therapy · 2025
    Review
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  19. Article
  20. Anti-Diabetic Effects of Ethanol Extract fromFoods (Basel, Switzerland) · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Xin Meng1Department of Ultrasonography, Xijing Hospital, the Fourth Military Medical University, Xi'an, 710032 Shaannxi China.
Minjuan Zheng1Department of Ultrasonography, Xijing Hospital, the Fourth Military Medical University, Xi'an, 710032 Shaannxi China.
Ming Yu1Department of Ultrasonography, Xijing Hospital, the Fourth Military Medical University, Xi'an, 710032 Shaannxi China.
Wei Bai1Department of Ultrasonography, Xijing Hospital, the Fourth Military Medical University, Xi'an, 710032 Shaannxi China.
Lei Zuo1Department of Ultrasonography, Xijing Hospital, the Fourth Military Medical University, Xi'an, 710032 Shaannxi China.
Xin Bu2Department of Biochemistry and Molecular Biology, the Fourth Military Medical University, Xi'an, 710032 Shaannxi China.
Yi Liu3Department of Cardiology, Xijing Hospital, the Fourth Military Medical University, Xi'an, 710032 Shaannxi China.
Linying Xia3Department of Cardiology, Xijing Hospital, the Fourth Military Medical University, Xi'an, 710032 Shaannxi China.
Jing Hu4Department of Radiation Oncology, Xijing Hospital, the Fourth Military Medical University, Xi'an, 710032 Shaannxi China.
Liwen Liu1Department of Ultrasonography, Xijing Hospital, the Fourth Military Medical University, Xi'an, 710032 Shaannxi China.
Jianping Li4Department of Radiation Oncology, Xijing Hospital, the Fourth Military Medical University, Xi'an, 710032 Shaannxi China.
Xijing Hospital · CNAir Force Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMyocardial infarction (MI) is a common cause of mortality in people. Mesenchymal stem cell (MSC) has been shown to exert therapeutic potential to treat myocardial infarction (MI). However, in patients with diabetes, the diabetic environment affected MSCs activity and could impair the efficacy of treatment. Interleukin-10 (IL-10) has been shown to attenuate MI by suppressing inflammation. In current study, the combination of MSC transplantation with IL-10 was evaluated in a diabetic mice model with MI.

methodsWe engineered bone marrow derived MSCs (BM-MSCs) to overexpress IL-10 by using CRISPR activation. We established the diabetic mice model with MI and monitored the IL-10 expression after BM-MSCs transplantation. We also evaluated the effects of BM-MSCs transplantation on inflammatory response, cell apoptosis, cardiac function and angiogenesis.

resultsCRISPR activation system enabled overexpression of IL-10 in BM-MSCs. Transplantation of BM-MSCs overexpressing IL-10 resulted in IL-10 expression in heart after transplantation. Transplantation of BM-MSCs overexpressing IL-10 inhibited inflammatory cell infiltration and pro-inflammatory cytokines production, improved cardiac functional recovery, alleviated cardiac injury, decreased apoptosis of cardiac cells and increased angiogenesis.

conclusionIn summary, we have demonstrated the therapeutic potential of IL-10 overexpressed BM-MSCs in the treatment of MI in diabetic mice.

Indexed as

BM-MSCsCRISPRaDiabeteIL-10Myocardial infarction

Identifiers

PMID31164920
PMCPMC6543626
OpenAlexW2947559070

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.