ArticleScientific reports2019
RDI Calculator: An Analysis Tool to Assess RNA Distributions in Cells.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- Reprogramming mRNA localization by targeted RNA-protein interference.bioRxiv : the preprint server for biology · 2026Article
- Cyclin CLB2 mRNA localization and protein synthesis link cell cycle progression to bud growth.Nature communications · 2025Article
- Control of epithelial tissue organization by mRNA localization.Nature communications · 2025Article
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- Microtubule-Mediated Regulation of βCirculation research · 2023Article
- mRNA location and translation rate determine protein targeting to dual destinations.Molecular cell · 2023Article
- Towards Understanding Long COVID: SARS-CoV-2 Strikes the Host Cell Nucleus.Pathogens (Basel, Switzerland) · 2023Review
- mRNA Location and Translation Rate Determine Protein Targeting to Dual Destinations.bioRxiv : the preprint server for biology · 2023Article
- Visualizing and Quantifying mRNA Localization at the Invasive Front of 3D Cancer Spheroids.Methods in molecular biology (Clifton, N.J.) · 2023Article
- RNA localization in confined cells depends on cellular mechanical activity and contributes to confined migration.iScience · 2022Article
- Article
- The kinesin KIF1C transports APC-dependent mRNAs to cell protrusions.RNA (New York, N.Y.) · 2021Article
- Interrogating RNA and protein spatial subcellular distribution in smFISH data with DypFISH.Cell reports methods · 2021Article
- Collective cancer cell invasion requires RNA accumulation at the invasive front.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- RNA localization and co-translational interactions control RAB13 GTPase function and cell migration.The EMBO journal · 2020Article
- Translational regulation of protrusion-localized RNAs involves silencing and clustering after transport.eLife · 2019Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Localization of RNAs to various subcellular destinations has emerged as a widely used mechanism that regulates a large proportion of transcripts in polarized cells. A number of methodologies have been developed that allow detection and imaging of RNAs at single-molecule resolution. However, methodologies to quantitatively describe RNA distributions are limited. Such approaches usually rely on the identification of cytoplasmic and nuclear boundaries which are used as reference points. Here, we describe an automated, interactive image analysis program that facilitates the accurate generation of cellular outlines from single cells and the subsequent calculation of metrics that quantify how a population of RNA molecules is distributed in the cell cytoplasm. We apply this analysis to mRNAs in mouse and human cells to demonstrate how these metrics can highlight differences in the distribution patterns of distinct RNA species. We further discuss considerations for the practical use of this tool. This program provides a way to facilitate and expedite the analysis of subcellular RNA localization for mechanistic and functional studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.