ArticleMethods and protocols2019
Real-Time In Vitro Fluorescence Anisotropy of the Cyanobacterial Circadian Clock.
Article in Methods and protocols, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- From phenomenon to mechanism in the circadian clock of Synechococcus elongatus.Npj biological timing and sleep · 2026Review
- Embrace Disorder as a Function: Bio-Informed Biomaterials Design Advanced by Intrinsically Disordered Proteins.Biomimetics (Basel, Switzerland) · 2026Article
- Circadian temperature compensation is intrinsically linked with metabolism and redox signaling.Nature communications · 2026Article
- Reconstitution of circadian clock in synthetic cells reveals principles of timekeeping.Nature communications · 2025Article
- Review
- Temperature-dependent fold-switching mechanism of the circadian clock protein KaiB.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Metamorphic proteins and how to find them.Current opinion in structural biology · 2024Review
- The inner workings of an ancient biological clock.Trends in biochemical sciences · 2024Review
- Circadian entrainment of in vitro reactions, in real time, and around the clock.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Synchronization of the circadian clock to the environment tracked in real time.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Dimer dissociation is a key energetic event in the fold-switch pathway of KaiB.Biophysical journal · 2022Article
- Reconstitution of an intact clock reveals mechanisms of circadian timekeeping.Science (New York, N.Y.) · 2021Article
- Monitoring Protein-Protein Interactions in the Cyanobacterial Circadian Clock in Real Time via Electron Paramagnetic Resonance Spectroscopy.Biochemistry · 2020Article
- Bayesian modeling reveals metabolite-dependent ultrasensitivity in the cyanobacterial circadian clock.Molecular systems biology · 2020Article
- Orchestration of Circadian Timing by Macromolecular Protein Assemblies.Journal of molecular biology · 2020Review
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Uniquely, the circadian clock of cyanobacteria can be reconstructed outside the complex milieu of live cells, greatly simplifying the investigation of a functioning biological chronometer. The core oscillator component is composed of only three proteins, KaiA, KaiB, and KaiC, and together with ATP they undergo waves of assembly and disassembly that drive phosphorylation rhythms in KaiC. Typically, the time points of these reactions are analyzed ex post facto by denaturing polyacrylamide gel electrophoresis, because this technique resolves the different states of phosphorylation of KaiC. Here, we describe a more sensitive method that allows real-time monitoring of the clock reaction. By labeling one of the clock proteins with a fluorophore, in this case KaiB, the in vitro clock reaction can be monitored by fluorescence anisotropy on the minutes time scale for weeks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.