ArticleMethods and protocols2019
Efficient Incorporation of Unnatural Amino Acids into Proteins with a Robust Cell-Free System.
Article in Methods and protocols, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- Bottom-Up Synthetic Biology Using Cell-Free Protein Synthesis.Advances in biochemical engineering/biotechnology · 2023Review
- Advancing synthetic biology through cell-free protein synthesis.Computational and structural biotechnology journal · 2023Review
- Toward efficient multiple-site incorporation of unnatural amino acids using cell-free translation system.Synthetic and systems biotechnology · 2022Article
- Article
- A linear DNA template-based framework for site-specific unnatural amino acid incorporation.Synthetic and systems biotechnology · 2021Article
- Development and comparison of cell-free protein synthesis systems derived from typical bacterial chassis.Bioresources and bioprocessing · 2021Article
- Physical stimuli-responsive cell-free protein synthesis.Synthetic and systems biotechnology · 2020Article
- Cell-free biology using remote-controlled digital microfluidics for individual droplet control.RSC advances · 2020Article
- Establishing a EukaryoticFrontiers in bioengineering and biotechnology · 2020Article
- "Cell-Free Synthetic Biology": Synthetic Biology Meets Cell-Free Protein Synthesis.Methods and protocols · 2019Article
- A User's Guide to Cell-Free Protein Synthesis.Methods and protocols · 2019Review
- Exploring the Potential of Cell-Free Protein Synthesis for Extending the Abilities of Biological Systems.Frontiers in bioengineering and biotechnology · 2019Review
- Advances and Challenges in Cell-Free Incorporation of Unnatural Amino Acids Into Proteins.Frontiers in pharmacology · 2019Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Unnatural proteins are crucial biomacromolecules and have been widely applied in fundamental science, novel biopolymer materials, enzymes, and therapeutics. Cell-free protein synthesis (CFPS) system can serve as a robust platform to synthesize unnatural proteins by highly effective site-specific incorporation of unnatural amino acids (UNAAs), without the limitations of cell membrane permeability and the toxicity of unnatural components. Here, we describe a quick and simple method to synthesize unnatural proteins in CFPS system based on
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.