Evidence map›Paper›PMID 31164584›Full record

ArticleMethods and protocols2018

A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria.

Siripat Ngoennet, Yasuhiro Nishikawa, Takashi Hibino, Rungaroon Waditee-Sirisattha, Hakuto Kageyama

Open access · goldAbstract read
In one paragraph

Article in Methods and protocols, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
6.2field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 49 citations in OpenAlex.

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  6. The Extract ofInternational journal of molecular sciences · 2024
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  15. Microalgae as a Source of Mycosporine-like Amino Acids (MAAs); Advances and Future Prospects.International journal of environmental research and public health · 2021
    Review
  16. Primary Assessment of Mycosporine-Like Amino Acids Production by Two Species ofIranian journal of pharmaceutical research : IJPR · 2021
    Article
  17. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Siripat NgoennetDepartment of Microbiology, Faculty of Science, Chulalongkorn University, Payathai Road, Pathumwan, Bangkok 10330, Thailand. siripat.ngoennet@gmail.com.
Yasuhiro NishikawaFaculty of Pharmacy, Meijo University, 150 Yagotoyama, Tenpaku-ku, Nagoya, Aichi 468-8503, Japan. yasuhiro@meijo-u.ac.jp.
Takashi HibinoFaculty of Science and Technology, Meijo University, 1-501 Shiogamaguchi, Tenpaku-ku, Nagoya, Aichi 468-8502, Japan. hibino@meijo-u.ac.jp.
Rungaroon Waditee-SirisatthaDepartment of Microbiology, Faculty of Science, Chulalongkorn University, Payathai Road, Pathumwan, Bangkok 10330, Thailand. Rungaroon.W@chula.ac.th.
Hakuto KageyamaFaculty of Science and Technology, Meijo University, 1-501 Shiogamaguchi, Tenpaku-ku, Nagoya, Aichi 468-8502, Japan. kageyama@meijo-u.ac.jp.ORCID 0000-0002-7741-7408
Meijo University · JPChulalongkorn University · TH

Funding

Sumitomo Foundation 170072
6 · The paper itself

Abstract

This report provides a broadly applicable and cost-effective method for the purification of mycosporine-like amino acids (MAAs) from cyanobacteria. As MAAs are known to have multiple bioactivities for health and beauty, a universal isolation method of MAAs from biomass is attractive. In particular, the biomass of photosynthetic microorganisms such as cyanobacteria is of interest as a natural source of useful compound production, because of their photoautotrophic property. The method presented here is applicable for the isolation of mycosporine-2-glycine (M2G), which is a rare MAA produced in a halotolerant cyanobacterium. This method also allowed for the isolation of two of the most common MAAs, shinorine (SHI) and porphyra-334 (P334). A three-step separation process using low pressure liquid chromatography yielded purified MAAs, which were characterized by nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC/MS) analyses. The purified MAAs exhibited free radical scavenging activity in the 2,2'-azino-

Indexed as

mycosporine-2-glycinemycosporine-like amino acidsporphyra-334shinorine

Identifiers

PMID31164584
PMCPMC6481085
OpenAlexW2902472794

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.