ArticleMethods and protocols2018
Segmentation of Total Cell Area in Brightfield Microscopy Images.
Article in Methods and protocols, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 14 citations in OpenAlex.
- Cellular Morphometric Analysis (CellMorph)-a comprehensive imaging-based tool for quantifying cellular phenotype heterogeneity and dynamics across biological processes.The FEBS journal · 2026Article
- A deterministic method for quantifying spindle-shaped cells in noisy bright-field microscopy.Scientific reports · 2026Article
- In Situ Functionalization of Polar Polythiophene-Based Organic Electrochemical Transistor to Interface In Vitro Models.ACS applied materials & interfaces · 2024Article
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- Live cell microscopy: From image to insight.Biophysics reviews · 2022Review
- The Degradation of Hyaluronan in the Skin.Biomolecules · 2022Article
- The ImageJ ecosystem: Open-source software for image visualization, processing, and analysis.Protein science : a publication of the Protein Society · 2021Article
- A Clonogenic Assay to Quantify Melanoma Micrometastases in Pulmonary Tissue.Methods in molecular biology (Clifton, N.J.) · 2021Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Segmentation is one of the most important steps in microscopy image analysis. Unfortunately, most of the methods use fluorescence images for this task, which is not suitable for analysis that requires a knowledge of area occupied by cells and an experimental design that does not allow necessary labeling. In this protocol, we present a simple method, based on edge detection and morphological operations, that separates total area occupied by cells from the background using only brightfield channel image. The resulting segmented picture can be further used as a mask for fluorescence quantification and other analyses. The whole procedure is carried out in open source software Fiji.
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Registered trials
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