ArticleMethods and protocols2018
Genetic Transformation of Recalcitrant Cassava by Embryo Selection and Increased Hormone Levels.
Article in Methods and protocols, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- An efficient regeneration protocol through somatic embryogenesis and organogenesis for cassava (Plant biotechnology (Tokyo, Japan) · 2025Article
- Advancements and strategies of genetic improvement in cassava (Horticulture research · 2025Article
- An Anthocyanin-Based Visual Reporter System for Genetic Transformation and Genome Editing in Cassava.International journal of molecular sciences · 2024Article
- Article
- Comparative Transcriptomics of Non-Embryogenic and Embryogenic Callus in Semi-Recalcitrant and Non-Recalcitrant Upland Cotton Lines.Plants (Basel, Switzerland) · 2021Article
- Current Advancements and Limitations of Gene Editing in Orphan Crops.Frontiers in plant science · 2021Review
- Efficient Genetic Transformation and Regeneration of a Farmer-Preferred Cassava Cultivar From Ghana.Frontiers in plant science · 2021Article
- A cassava protoplast system for screening genes associated with the response to South African cassava mosaic virus.Virology journal · 2020Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Genetic engineering is considered to be an important tool for the improvement of cassava. Cassava is a highly heterozygous crop species for which conventional breeding is a lengthy and tedious process. Robust transformation is based on Agrobacterium-mediated transformation of friable embryogenic callus (FEC). Production of FEC is genotype-dependent and considered to be a major bottleneck for the genetic transformation of cassava. As a consequence, routine genetic transformation has only been established for a handful of cassava cultivars. Therefore, development of procedures enabling efficient production of high-quality cassava FEC is required to allow the translation of research from the model cultivar to farmer-preferred cassava cultivars. Here we study the FEC production capacity of Brazilian cassava cultivars and report the modification of the protocol for the genetic transformation of Verdinha (BRS 222), a recalcitrant cultivar with high potential for protein production that is extensively used by farmers in Brazil.
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Registered trials
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