ArticleMethods and protocols2018
Build Your Own Microscope: Step-By-Step Guide for Building a Prism-Based TIRF Microscope.
Article in Methods and protocols, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- A Standardized Prism-Based TIRF Platform for Quantitative Single-Molecule Fluorescence Studies of Biomolecular Dynamics.Biosensors · 2026Article
- Multiplexed Single-Molecule Detection of Nucleic Acid Biomarkers with a Distance-Tuned Single FRET Pair.Analytical chemistry · 2025Article
- Integrating FRET and Molecular Dynamics Simulation for Single-Molecule Aptameric Detection of Staphylococcus aureus IsdA Surface Protein.Biotechnology journal · 2025Article
- Structural Dynamics of SNARE Complex Assembly in the Ribbon Synapses Observed by smFRET.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Exploring GPCR conformational dynamics using single-molecule fluorescence.Methods (San Diego, Calif.) · 2024Review
- Single-Molecule FRET-Based Multiplexed Detection.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Multiplexed smFRET Nucleic Acid Sensing Using DNA Nanotweezers.Biosensors · 2023Article
- Using Single-Molecule FRET to Evaluate DNA Nanodevices at Work.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Probing Transient Riboswitch Structures via Single Molecule Accessibility Analysis.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Optical tweezer and TIRF microscopy for single molecule manipulation of RNA/DNA nanostructures including their rubbery property and single molecule counting.Biophysics reports · 2021Article
- Fast-dissociating but highly specific antibodies are novel tools in biology, especially useful for multiplex super-resolution microscopy.STAR protocols · 2021Article
- Optometry for a short-sighted microscope.Biophysical journal · 2021Article
- Construction of a Three-Color Prism-Based TIRF Microscope to Study the Interactions and Dynamics of Macromolecules.Biology · 2021Article
- Single-Molecule FRET-Based Dynamic DNA Sensor.ACS sensors · 2021Article
- A Systematic, Open-Science Framework for Quantification of Cell-Types in Mouse Brain Sections Using Fluorescence Microscopy.Frontiers in neuroanatomy · 2021Article
- FRET-Based Aptasensor for the Selective and Sensitive Detection of Lysozyme.Sensors (Basel, Switzerland) · 2020Article
- Single-molecule analysis of i-motif within self-assembled DNA duplexes and nanocircles.Nucleic acids research · 2019Article
- Single-Step FRET-Based Detection of Femtomoles DNA.Sensors (Basel, Switzerland) · 2019Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Prism-based total internal reflection fluorescence (pTIRF) microscopy is one of the most widely used techniques for the single molecule analysis of a vast range of samples including biomolecules, nanostructures, and cells, to name a few. It allows for excitation of surface bound molecules/particles/quantum dots via evanescent field of a confined region of space, which is beneficial not only for single molecule detection but also for analysis of single molecule dynamics and for acquiring kinetics data. However, there is neither a commercial microscope available for purchase nor a detailed guide dedicated for building this microscope. Thus far, pTIRF microscopes are custom-built with the use of a commercially available inverted microscope, which requires high level of expertise in selecting and handling sophisticated instrument-parts. To directly address this technology gap, here we describe a step-by-step guide on how to build and characterize a pTIRF microscope for in vitro single-molecule imaging, nanostructure analysis and other life sciences research.
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