ArticleMethods and protocols2018
Two-Step Concentration of Complex Water Samples for the Detection of Viruses.
Article in Methods and protocols, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Detection of echovirus 7 in effluent sewage treatment plant Ahvaz city, Iran.Iranian journal of microbiology · 2026Article
- The potential of long-term wastewater-based surveillance to predict COVID-19 waves peak in Mexico.Water environment research : a research publication of the Water Environment Federation · 2025Article
- Review
- Viruses in Wastewater-A Concern for Public Health and the Environment.Microorganisms · 2024Review
- Improved virus concentration methods for wash waters from decontamination of permeable and non-permeable surfaces.Journal of virological methods · 2023Article
- Influence of membrane pore-size on the recovery of endogenous viruses from wastewater using an adsorption-extraction method.Journal of virological methods · 2023Article
- Evaluating the transmission risk of SARS-CoV-2 from sewage pollution.The Science of the total environment · 2023Article
- Article
- Occurrence and Accumulation of Human Enteric Viruses and Phages in Process Water from the Fresh Produce Industry.Foods (Basel, Switzerland) · 2021Article
- Monitoring SARS-CoV-2 in municipal wastewater to evaluate the success of lockdown measures for controlling COVID-19 in the UK.Water research · 2021Article
- Viruses in wastewater: occurrence, abundance and detection methods.The Science of the total environment · 2020Review
- Sewage analysis as a tool for the COVID-19 pandemic response and management: the urgent need for optimised protocols for SARS-CoV-2 detection and quantification.Journal of environmental chemical engineering · 2020Article
- Integrated Electrochemical Biosensors for Detection of Waterborne Pathogens in Low-Resource Settings.Biosensors · 2020Review
- Critical Evaluation of CrAssphage as a Molecular Marker for Human-Derived Wastewater Contamination in the Aquatic Environment.Food and environmental virology · 2019Article
- Seasonal and diurnal surveillance of treated and untreated wastewater for human enteric viruses.Environmental science and pollution research international · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
The accurate detection and quantification of pathogenic viruses in water is essential to understand and reduce the risk of human infection. This paper describes a two-step method suitable for concentrating viruses in water and wastewater samples. The method involves a tangential flow ultrafiltration step that reduces the sample volume of 1-10 L to approximately 50 mL, followed by secondary precipitation using polyethylene glycol 6000, which reduces the volume to 1-4 mL. For method validation, water samples were spiked with different concentrations of enteric viruses, and viral recovery in the concentrates exceeded 10% in all experiments. The method is suitable for water samples with high and low salinity and turbidity, allowing an accurate comparison of viral titers in a diverse range of water types. Furthermore, the method has the potential to concentrate other pathogens, e.g., bacteria or protozoa. Hence, the use of this method can improve the holistic assessment of risks associated with wastewater-contaminated environments.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.