Evidence map›Paper›PMID 31162576›Full record

ArticleThe Journal of nutrition2019

Early-Life Iron Deficiency Alters Glucose Transporter-1 Expression in the Adult Rodent Hippocampus.

Kathleen Ennis, Barbara Felt, Michael K Georgieff, Raghavendra Rao

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of nutrition, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.2field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 11 citations in OpenAlex.

  1. Trial
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Nutrition and the developing brain.Pediatric research · 2020
    Article
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Kathleen EnnisDivision of Neonatology, Department of Pediatrics, University of Minnesota, Minneapolis, MN, USA.
Barbara FeltDepartment of Pediatrics, University of Michigan, Ann Arbor, MI, USA.
Michael K GeorgieffDivision of Neonatology, Department of Pediatrics, University of Minnesota, Minneapolis, MN, USA.
Raghavendra RaoDivision of Neonatology, Department of Pediatrics, University of Minnesota, Minneapolis, MN, USA.
University of Minnesota · USUniversity of Michigan · US

Funding

STATISTICAL COREP01HD039386 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI COE, CHRISTOPHER · 2001 to 2011
$14.0M
A Genetic Model of Perinatal Hippocampal Iron DeficiencyR21HD054490 · NICHD · UNIVERSITY OF MINNESOTA · PI GEORGIEFF, MICHAEL K. · 2007 to 2009
$424k
NICHD NIH HHS P01 HD039386NICHD NIH HHS R21 HD054490
6 · The paper itself

Abstract

backgroundEarly-life iron deficiency (ID) impairs hippocampal energy production. Whether there are changes in glucose transporter (GLUT) expression is not known.

objectiveThe aim of this study was to investigate whether early-life ID and the treatment iron dose alter brain regional GLUT expression in adult rats and mice.

methodsIn Study 1, ID was induced in male and female Sprague Dawley rat pups by feeding dams a 3-mg/kg iron diet during gestation and the first postnatal week, followed by treatment using low-iron [3-10 mg/kg; formerly iron-deficient (FID)-10 group], standard-iron (40-mg/kg; FID-40 group), or high-iron (400-mg/kg; FID-400 group) diets until weaning. The control group received the 40 mg/kg iron diet. GLUT1, GLUT3, hypoxia-inducible factor (HIF)-1α, and prolyl-hydroxylase-2 (PHD2) mRNA and protein expression in the cerebral cortex, hippocampus, striatum, cerebellum, and hypothalamus were determined at adulthood. In Study 2, the role of hippocampal ID in GLUT expression was examined by comparing the Glut1, Glut3, Hif1α, and Phd2 mRNA expression in adult male and female wild-type (WT) and nonanemic hippocampal iron-deficient and iron-replete dominant negative transferrin receptor 1 (DNTfR1-/-) transgenic mice.

resultsIn Study 1, Glut1, Glut3, and Hif1α mRNA, and GLUT1 55-kDa protein expression was upregulated 20-33% in the hippocampus of the FID-10 group but not the FID-40 group, relative to the control group. Hippocampal Glut1 mRNA (-39%) and GLUT1 protein (-30%) expression was suppressed in the FID-400 group, relative to the control group. Glut1 and Glut3 mRNA expression was not altered in the other brain regions in the 3 FID groups. In Study 2, hippocampal Glut1 (+14%) and Hif1α (+147%) expression was upregulated in the iron-deficient DNTfR1-/- mice, but not in the iron-replete DNTfR1-/- mice, relative to the WT mice (P < 0.05, all).

conclusionsEarly-life ID is associated with altered hippocampal GLUT1 expression in adult rodents. The mouse study suggests that tissue ID is potentially responsible.

Indexed as

Iron DeficienciesAnimalsFemaleGlucose Transporter Type 1Glucose Transporter Type 3HippocampusHypoxia-Inducible Factor 1, alpha SubunitMaleMiceRatsRats, Sprague-DawleyReceptors, TransferrinRNA, MessengerGlucose Transporter Type 1Glucose Transporter Type 3Hif1a protein, ratHypoxia-Inducible Factor 1, alpha SubunitReceptors, TransferrinRNA, MessengerSlc2a1 protein, ratSlc2a3 protein, ratglucose transporter-1glucose transporter-3hippocampushypoxia-inducible factoriron deficiencyiron toxicityprolyl-hydroxylasetransferrin receptor

Identifiers

PMID31162576
PMCPMC6736205
OpenAlexW2948203983

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.