ArticleChemical science2019
A H-bond strategy to develop acid-resistant photoswitchable rhodamine spirolactams for super-resolution single-molecule localization microscopy.
Article in Chemical science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 105 citations in OpenAlex.
- Fluorogenic rhodamine B derivatives that become brighter at neutral pH.Bioorganic & medicinal chemistry letters · 2026Article
- A Photoswitchable HaloTag for Spatiotemporal Control of Fluorescence in Living Cells.Angewandte Chemie (International ed. in English) · 2025Article
- Synthetic fluorophores for live-cell fluorescence microscopy and biosensing.Nature chemical biology · 2025Review
- Photoswitchable Fluorescent Hydrazone for Super-Resolution Cell Membrane Imaging.Journal of the American Chemical Society · 2025Article
- The ATTO 565 Dye and Its Applications in Microscopy.Molecules (Basel, Switzerland) · 2024Review
- Squaraine Dyes Exhibit Spontaneous Fluorescence Blinking That Enables Live-Cell Nanoscopy.Nano letters · 2024Article
- Photochemical Mechanisms of Fluorophores Employed in Single-Molecule Localization Microscopy.Angewandte Chemie (International ed. in English) · 2023Review
- pH-Responsive Drug Delivery and Imaging Study of Hybrid Mesoporous Silica Nanoparticles.Molecules (Basel, Switzerland) · 2022Article
- Gadolinium-Coated Mesoporous Silica Nanoparticle for Magnetic Resonance Imaging.Frontiers in chemistry · 2022Article
- Stabilizing the framework of SAPO-34 zeolite toward long-term methanol-to-olefins conversion.Nature communications · 2021Article
- Directed transforming of coke to active intermediates in methanol-to-olefins catalyst to boost light olefins selectivity.Nature communications · 2021Article
- Recent Progress in Small Spirocyclic, Xanthene-Based Fluorescent Probes.Molecules (Basel, Switzerland) · 2020Review
- Imaging spatiotemporal evolution of molecules and active sites in zeolite catalyst during methanol-to-olefins reaction.Nature communications · 2020Article
- Visualizing semipermeability of the cell membrane using a pH-responsive ratiometric AIEgen.Chemical science · 2020Article
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Corrections and comments
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Authors and funding
13 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rhodamine spirolactam based photoswitches have been extensively applied in super-resolution single-molecule localization microscopy (SMLM). However, the ring-opening reactions of spirolactams are cross-sensitive to acid, limiting their photoswitch use to neutral pH conditions. In addition, the ring-closing reactions of spirolactams are environment-sensitive and slow (up to hours), virtually making rhodamine spirolactams caged fluorescent dyes instead of reversible photoswitches in SMLM. Herein, by introducing hydrogen bonds to stabilize spirolactams, we report a series of acid-resistant rhodamine spirolactams with accelerated ring-closing reactions from fluorescent xanthyliums to non-fluorescent spirolactams, endowing them with good photoswitchable properties even in acidic environments. By further substitution of 6-phenylethynyl naphthalimide on the spirolactam, we shifted the photoactivation wavelength into the visible region (>400 nm). Subsequently, we have successfully applied these dyes in labeling and imaging the cell surface of
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