ArticleChemical science2019
Genome mining and biosynthesis of kitacinnamycins as a STING activator.
Article in Chemical science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Genomic, biological, and chemical studies of Streptomyces sp. LaBMicrA B280 isolated from the rhizosphere of Inga edulis Martius in the Amazon.Antonie van Leeuwenhoek · 2026Article
- High-Throughput Prediction of Protein-Protein Interactions Uncovers Hidden Molecular Networks in Biosynthetic Gene Clusters.Computational and structural biotechnology journal · 2026Article
- Logical Exploration of Cinnamoyl-Containing Nonribosomal Peptides via Metabologenomic Targeting and Regulator Overexpression.Journal of the American Chemical Society · 2025Article
- Synthetic-bioinformatic natural product-inspired peptides.Natural product reports · 2025Review
- Biosynthesis and genome mining strategies for purine-derivedFrontiers in microbiology · 2025Review
- Cinnamosyn, a Cinnamoylated Synthetic-Bioinformatic Natural Product with Cytotoxic Activity.Organic letters · 2024Article
- OSMAC-Based Discovery and Biosynthetic Gene Clusters Analysis of Secondary Metabolites from Marine-DerivedMarine drugs · 2023Article
- Mitochondrial DNA-triggered innate immune response: mechanisms and diseases.Cellular & molecular immunology · 2023Review
- Genomic Based Analysis of the Biocontrol SpeciesJournal of fungi (Basel, Switzerland) · 2023Article
- Absolute Stereochemistry Determination of Bioactive Marine-Derived Cyclopeptides by Liquid Chromatography Methods: An Update Review (2018-2022).Molecules (Basel, Switzerland) · 2023Review
- Resistance and phylogeny guided discovery reveals structural novelty of tetracycline antibiotics.Chemical science · 2022Article
- Epoxinnamide: An Epoxy Cinnamoyl-Containing Nonribosomal Peptide from an Intertidal Mudflat-DerivedMarine drugs · 2022Article
- Youssoufenes A2 and A3, Antibiotic Dimeric Cinnamoyl Lipids from the ΔMarine drugs · 2022Article
- Production of chain-extended cinnamoyl compounds by overexpressing two adjacent cluster-situated LuxR regulators inFrontiers in microbiology · 2022Article
- Renewed interests in the discovery of bioactive actinomycete metabolites driven by emerging technologies.Journal of applied microbiology · 2022Review
- Genome mining methods to discover bioactive natural products.Natural product reports · 2021Review
- Antifungal Activity and Biosynthetic Potential of NewInternational journal of molecular sciences · 2021Article
- Putative hexameric glycosyltransferase functional unit revealed by the crystal structure ofIUCrJ · 2021Article
- Therapeutic Development by Targeting the cGAS-STING Pathway in Autoimmune Disease and Cancer.Frontiers in pharmacology · 2021Review
- Discovery and biosynthesis of bosamycins fromChemical science · 2020Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cinnamoyl-containing nonribosomal peptides (CCNPs) are a small group of secondary metabolites with potent biological activities produced by actinobacteria. Two remarkable features in the biosynthesis of CCNPs include the nonribosomal peptide synthases (NRPSs) for assembly of the depsipeptide backbone and the type II polyketide synthases (PKSs) for N-terminal cinnamoyl moiety construction. Here, we present a genome mining approach targeting both NRPS and type II PKS for discovery of new CCNPs, which led to the identification of 51 putative CCNP gene clusters from public bacterial genome databases. After strain prioritization, a novel class of CCNP-type glycopeptides named kitacinnamycins, one of which showing potent activation ability towards the stimulator of interferon genes (STING) protein, was identified. Bioinformatic, genetic and biochemical analysis revealed the use of the NRPS assembly line to form the macrocyclic peptide backbone, followed by a P450 monooxygenase to generate terminal oxidized groups. A glycosyltransferase with relatively broad substrate specificity transfers sugars to the newly generated OH/COOH group. The protein crystallographic study further provided structural insights into this glycosylation. Our results not only demonstrated the feasibility of genome mining and strain prioritization for the discovery of new bioactive natural products but also disclosed the biosynthetic pathway for kitacinnamycins.
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Registered trials
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