ArticleNature protocols2019
Transgenesis in Hydra to characterize gene function and visualize cell behavior.
Article in Nature protocols, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Efficient genome editing using CRISPR-Cas9 in reef-building corals.Nature protocols · 2026Review
- A molecular, spatial and regulatory atlas of the Hydra vulgaris nervous system.Development (Cambridge, England) · 2025Article
- Assembly of a functional neuronal circuit in embryos of an ancestral metazoan is influenced by temperature and the microbiome.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Novel technologies uncover novel 'anti'-microbial peptides inPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Article
- Automatic monitoring of neural activity with single-cell resolution in behaving Hydra.Scientific reports · 2024Article
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- A chromosome-scale epigenetic map of theGenome research · 2023Article
- Harnessing the Power of Model Organisms To Unravel Microbial Functions in the Coral Holobiont.Microbiology and molecular biology reviews : MMBR · 2022Review
- Articulating the "stem cell niche" paradigm through the lens of non-model aquatic invertebrates.BMC biology · 2022Review
- Gene Manipulation in Hydractinia.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Cell polarity signalling at the birth of multicellularity: What can we learn from the first animals.Frontiers in cell and developmental biology · 2022Review
- Cellular, Metabolic, and Developmental Dimensions of Whole-Body Regeneration inCold Spring Harbor perspectives in biology · 2021Review
- Article
- Epigenetic Regulation inFrontiers in cell and developmental biology · 2021Review
- Prototypical pacemaker neurons interact with the resident microbiota.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Electric-Induced Reversal of Morphogenesis in Hydra.Biophysical journal · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The freshwater polyp Hydra is a cnidarian used as a model organism in a number of fields, including the study of the origin and evolution of developmental mechanisms, aging, symbiosis and host-microbe interactions. Here, we describe a procedure for the establishment of stable transgenic Hydra lines by embryo microinjection. The three-stage protocol comprises (i) the design and preparation of a transgenic construct, (ii) the microinjection of the vector into early embryos of Hydra vulgaris, and (iii) the selection and enrichment of mosaic animals in order to develop uniformly transgenic clonal lines. The preparation of a transgenic construct requires ~2 weeks, and transgenic lines can be obtained within 3 months. The method allows constitutive or inducible gain- and loss-of-function approaches, as well as in vivo tracing of individual cells. Hydra polyps carrying transgenic cells reveal functional properties of the ancestral circuitry controlling animal development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.