ArticleNature cell biology2019
Establishment of porcine and human expanded potential stem cells.
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Article in Nature cell biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 213 papers.
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213citing papers in PubMed
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213 citing papers in PubMed.
- One-Step Induction of Oogonia-Like and Pro-Spermatogonia-Like Cells From Porcine Pluripotent Stem Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Livestock Blastoids: In Vitro Blastocyst Models for Embryogenesis and Reproductive Technologies.Reproduction in domestic animals = Zuchthygiene · 2026Review
- Suidae iPSC-derived macrophages as models for investigating susceptibility and resilience to African swine fever virus.Stem cell reports · 2026Article
- The making of cells and organs in xenogeneic animal bioreactor.Signal transduction and targeted therapy · 2026Review
- Generation of modified cows and sheep from spermatid-like haploid embryonic stem cells.Nature biotechnology · 2026Article
- Article
- Article
- Enhancing reprogramming towards induced human expanded pluripotency through substitution of SOX2 with engineered SOX17 transcription factors.Communications biology · 2026Article
- Efficient derivation of stable sheep embryonic stem cells opens a new avenue for agricultural and biomedical application.Journal of advanced research · 2026Article
- Models of the human heart for biomedical research: Opportunities and challenges.Physiological reports · 2026Review
- HAND1 controls the lineage bifurcation of trophoblast and amnion from human pluripotent stem cells.Cellular and molecular life sciences : CMLS · 2026Article
- Generating high-quality porcine iPSCs with the new medium cocktail LACID.Stem cell reports · 2026Article
- Progress in stem cell-based embryo models and their applications in developmental biology and biomedicine.Nature reviews. Molecular cell biology · 2026Review
- Single-Cell Mitochondrial Lineage Tracing Decodes Fate Decision and Spatial Clonal Architecture in Human Hematopoietic Organoids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Amniogenesis in embryos and stem cell models.Nature cell biology · 2026Review
- Wnt/β-Catenin Activation by iCRT3 Enhanced the Pluripotency of Bovine Expanded Pluripotent Stem Cells.Animals : an open access journal from MDPI · 2026Article
- Trophectoderm-like cells from EPS cells enable generating EPS cell-derived post-implantation embryoids that complete gastrulation.Protein & cell · 2026Article
- In vitro mimicking of humanized cardiogenesis under porcine condition.Cell & bioscience · 2026Article
- Comparative transcriptomic analysis of embryonic stem cells across mammalian species.iScience · 2026Article
- Review
153 more citing papers are in PubMed but not listed here.
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Authors and funding
47 authors.
Xuefei GaoSchool of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Stem Cell and Regenerative Medicine Consortium, Pokfulam, Hong Kong.
Monika Nowak-ImialekInstitute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Mariensee, Neustadt, Germany.
Xi ChenThe Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.ORCID http://orcid.org/0000-0003-2648-3146
Dongsheng ChenBGI-Shenzhen, Shenzhen, China.
Doris HerrmannInstitute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Mariensee, Neustadt, Germany.
Degong RuanSchool of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Stem Cell and Regenerative Medicine Consortium, Pokfulam, Hong Kong.
Andy Chun Hang ChenDepartment of Obstetrics and Gynaecology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong.
Melanie A Eckersley-MaslinEpigenetics Programme, Babraham Institute, Babraham Research Campus, Cambridge, UK.
Shakil AhmadAston Medical Research Institute, Aston Medical School, Aston University, Birmingham, UK.
Yin Lau LeeDepartment of Obstetrics and Gynaecology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong.
Toshihiro KobayashiWellcome Trust and Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK.ORCID http://orcid.org/0000-0001-8019-0008
David RyanThe Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.
Jixing ZhongBGI-Shenzhen, Shenzhen, China.
Jiacheng ZhuBGI-Shenzhen, Shenzhen, China.
Jian WuSchool of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Stem Cell and Regenerative Medicine Consortium, Pokfulam, Hong Kong.
Guocheng LanCancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.ORCID http://orcid.org/0000-0001-9063-5728
Stoyan PetkovInstitute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Mariensee, Neustadt, Germany.
Jian YangThe Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.
Liliana AntunesThe Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.
Lia S CamposThe Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.
Beiyuan FuThe Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.
Shengpeng WangBGI-Shenzhen, Shenzhen, China.
Yu YongThe Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.
Xiaomin WangKey Laboratory of Regenerative Biology of Chinese Academy of Sciences, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.
Song-Guo XueCenter for Reproductive Medicine, Shanghai East Hospital, School of Medicine, Tong Ji University, Shanghai, China.
Liangpeng GeChongqing Academy of Animal Sciences and Key Laboratory of Pig Industry Sciences, Department of Agriculture, Chongqing, China.
Zuohua LiuChongqing Academy of Animal Sciences and Key Laboratory of Pig Industry Sciences, Department of Agriculture, Chongqing, China.
Yong HuangChongqing Academy of Animal Sciences and Key Laboratory of Pig Industry Sciences, Department of Agriculture, Chongqing, China.
Tao NieKey Laboratory of Regenerative Biology of Chinese Academy of Sciences, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.
Peng LiKey Laboratory of Regenerative Biology of Chinese Academy of Sciences, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.ORCID http://orcid.org/0000-0003-4530-2400
Donghai WuKey Laboratory of Regenerative Biology of Chinese Academy of Sciences, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.
Duanqing PeiKey Laboratory of Regenerative Biology of Chinese Academy of Sciences, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.
Yi ZhangBiotherapy Center, The First Affiliated Hospital of Zhengzhou University, Henan, China.ORCID http://orcid.org/0000-0001-9861-4681
Liming LuInstitute of Immunology, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
Fengtang YangThe Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.ORCID http://orcid.org/0000-0002-3573-2354
Susan J KimberFaculty of Biology Medicine and Health, University of Manchester, Manchester, UK.
Wolf ReikEpigenetics Programme, Babraham Institute, Babraham Research Campus, Cambridge, UK.
Xiangang ZouCancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.
Zhouchun ShangBGI-Shenzhen, Shenzhen, China.ORCID http://orcid.org/0000-0002-1740-7961
Liangxue LaiKey Laboratory of Regenerative Biology of Chinese Academy of Sciences, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.
Azim SuraniWellcome Trust and Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK.ORCID http://orcid.org/0000-0002-8640-4318
Patrick P L TamEmbryology Unit, Children's Medical Research Institute and School of Medical Sciences, Sydney Medical School, Faculty of Medicine and Health, The University of Sydney, Westmead, NSW, Australia.ORCID http://orcid.org/0000-0001-6950-8388
Asif AhmedAston Medical Research Institute, Aston Medical School, Aston University, Birmingham, UK.
William Shu Biu YeungDepartment of Obstetrics and Gynaecology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong.
Sarah A TeichmannThe Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.ORCID http://orcid.org/0000-0002-6294-6366
Heiner NiemannInstitute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Mariensee, Neustadt, Germany. niemann.heiner@mh-hannover.de.ORCID http://orcid.org/0000-0003-0282-9704
Pentao LiuSchool of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Stem Cell and Regenerative Medicine Consortium, Pokfulam, Hong Kong. pliu88@hku.hk.ORCID http://orcid.org/0000-0001-5774-9678
Funding
Cancer Research UK A24843Medical Research Council G0700288Medical Research Council G0801057Medical Research Council MR/M017354/1Wellcome Trust 095645Wellcome Trust 098051Wellcome Trust 203144
Abstract
We recently derived mouse expanded potential stem cells (EPSCs) from individual blastomeres by inhibiting the critical molecular pathways that predispose their differentiation. EPSCs had enriched molecular signatures of blastomeres and possessed developmental potency for all embryonic and extra-embryonic cell lineages. Here, we report the derivation of porcine EPSCs, which express key pluripotency genes, are genetically stable, permit genome editing, differentiate to derivatives of the three germ layers in chimeras and produce primordial germ cell-like cells in vitro. Under similar conditions, human embryonic stem cells and induced pluripotent stem cells can be converted, or somatic cells directly reprogrammed, to EPSCs that display the molecular and functional attributes reminiscent of porcine EPSCs. Importantly, trophoblast stem-cell-like cells can be generated from both human and porcine EPSCs. Our pathway-inhibition paradigm thus opens an avenue for generating mammalian pluripotent stem cells, and EPSCs present a unique cellular platform for translational research in biotechnology and regenerative medicine.
Indexed as
AnimalsBlastomeresCell DifferentiationCell LineageCellular ReprogrammingEmbryonic Stem CellsGerm LayersHumansInduced Pluripotent Stem CellsMicePluripotent Stem CellsRegenerative MedicineSignal TransductionSwineTrophoblasts
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