ArticleScientific reports2019
Characterization of mouse ocular response to a 35-day spaceflight mission: Evidence of blood-retinal barrier disruption and ocular adaptations.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- AstroOphthalmology: Basic and Pathophysiological Concepts of Space-Induced Ocular Changes.Eye and brain · 2026Review
- A systematic review of the cerebrovascular adaptations following exposure to spaceflight or ground-based analogs: lessons from human and animal studies.NPJ microgravity · 2025Article
- NMN Supplementation Inhibits Endothelial Cell ROS-Mediated Src/Pi3k/Akt Signaling Pathway to Protect High-Altitude Blood-Retinal Barrier.Investigative ophthalmology & visual science · 2025Article
- Intraocular Pressure and Retinal Nerve Fiber Layer Changes in a Microgravity Mouse Model and Relevance to Spaceflight-Associated Neuro-Ocular Syndrome.Eye and brain · 2025Article
- Impact of preoperative intraocular pressure on corneal endothelial cell loss after phacoemulsification in acute primary angle-closure glaucoma with cataracts.Frontiers in medicine · 2025Article
- Artificial Gravity Attenuates the Transcriptomic Response to Spaceflight in the Optic Nerve and Retina.International journal of molecular sciences · 2024Article
- Spaceflight increases sarcoplasmic reticulum CaNPJ microgravity · 2024Article
- Evidence of Spaceflight-Induced Adverse Effects on Photoreceptors and Retinal Function in the Mouse Eye.International journal of molecular sciences · 2023Article
- Review
- Review
- Astrocytes regulate vascular endothelial responses to simulated deep space radiation in a human organ-on-a-chip model.Frontiers in immunology · 2022Article
- Evaluating Ocular Response in the Retina and Optic Nerve Head after Single and Fractionated High-Energy Protons.Life (Basel, Switzerland) · 2021Article
- Spaceflight decelerates the epigenetic clock orchestrated with a global alteration in DNA methylome and transcriptome in the mouse retina.Precision clinical medicine · 2021Article
- Spaceflight and hind limb unloading induces an arthritic phenotype in knee articular cartilage and menisci of rodents.Scientific reports · 2021Article
- Suprabasin-A Review.Genes · 2021Review
- The individual and combined effects of spaceflight radiation and microgravity on biologic systems and functional outcomes.Journal of environmental science and health. Part C, Toxicology and carcinogenesis · 2021Article
- Spaceflight induces oxidative damage to blood-brain barrier integrity in a mouse model.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Integrated RNA-seq Analysis Indicates Asynchrony in Clock Genes between Tissues under Spaceflight.Life (Basel, Switzerland) · 2020Article
- Validation of a New Rodent Experimental System to Investigate Consequences of Long Duration Space Habitation.Scientific reports · 2020Article
- Guest Edited Collection: Gravitational biology and space medicine.Scientific reports · 2019Article
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13 authors.
Funding
Abstract
The health risks associated with spaceflight-induced ocular structural and functional damage has become a recent concern for NASA. The goal of the present study was to characterize the effects of spaceflight and reentry to 1 g on the structure and integrity of the retina and blood-retinal barrier (BRB) in the eye. To investigate possible mechanisms, changes in protein expression profiles were examined in mouse ocular tissue after spaceflight. Ten week old male C57BL/6 mice were launched to the International Space Station (ISS) on Space-X 12 at the Kennedy Space Center (KSC) on August, 2017. After a 35-day mission, mice were returned to Earth alive. Within 38 +/- 4 hours of splashdown, mice were euthanized and ocular tissues were collected for analysis. Ground control (GC) and vivarium control mice were maintained on Earth in flight hardware or normal vivarium cages respectively. Repeated intraocular pressure (IOP) measurements were performed before the flight launch and re-measured before the mice were euthanized after splashdown. IOP was significantly lower in post-flight measurements compared to that of pre-flight (14.4-19.3 mmHg vs 16.3-20.3 mmHg) (p < 0.05) for the left eye. Flight group had significant apoptosis in the retina and retinal vascular endothelial cells compared to control groups (p < 0.05). Immunohistochemical analysis of the retina revealed that an increased expression of aquaporin-4 (AQP-4) in the flight mice compared to controls gave strong indication of disturbance of BRB integrity. There were also a significant increase in the expression of platelet endothelial cell adhesion molecule-1 (PECAM-1) and a decrease in the expression of the BRB-related tight junction protein, Zonula occludens-1 (ZO-1). Proteomic analysis showed that many key proteins and pathways responsible for cell death, cell cycle, immune response, mitochondrial function and metabolic stress were significantly altered in the flight mice compared to ground control animals. These data indicate a complex cellular response that may alter retina structure and BRB integrity following long-term spaceflight.
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