ArticleNature communications2019
Terminator-free template-independent enzymatic DNA synthesis for digital information storage.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers.
What it found
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Who cites it
91 citing papers in PubMed.
- Substrate energetics in non-templated DNA synthesis by terminal deoxynucleotidyl transferase.Nucleic acids research · 2026Article
- Programmable one-pot polymerase-mediated DNA synthesis via temperature control.Nature communications · 2026Article
- Engineering a Virus-Derived X Family DNA Polymerase FvPolX for de novo DNA Synthesis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- ZAT-DNA enables DNA data storage with molecular-layer non-replicability.Nature communications · 2026Article
- Comparison of state-of-the-art error-correction coding for sequence-based DNA data storage.Nature communications · 2026Article
- Approaching single-molecule assembly-free readout from medium-length encoded DNA.Nature communications · 2025Article
- Highly Ordered DNA Framework Interface Enables Efficient Enzymatic Oligonucleotide Synthesis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- A compact cassette tape for DNA-based data storage.Science advances · 2025Article
- Achieving handle-level random access in an encrypted DNA archival storage system via frequency dictionary mapping coding.Patterns (New York, N.Y.) · 2025Article
- Advancing synthesis-free and enzyme-free rewritable DNA memory through frameshift encoding and nanopore duplex interruption decoding.PNAS nexus · 2025Article
- Integrating DNA-Based Memory in Water-Resistant Electrospun Polymer Fibers for Nondestructive Data Retrieval.ACS applied materials & interfaces · 2025Article
- Dna-storalator: a computational simulator for DNA data storage.BMC bioinformatics · 2025Article
- A Programmed DNA Dynamic Assembly-Guided Molecular Amplifier for Authentic Information Decryption.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Article
- Template-independent enzymatic functionalization of DNA oligonucleotides with environment-sensitive nucleotide probes using terminal deoxynucleotidyl transferase.Nucleic acids research · 2025Article
- Cost-Effective DNA Storage System with DNA Movable Type.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Terminal deoxynucleotidyl transferase: Properties and applications.Engineering microbiology · 2025Review
- Long oligos: direct chemical synthesis of genes with up to 1728 nucleotides.Chemical science · 2025Article
- Biotechnological tools boost the functional diversity of DNA-based data storage systems.Computational and structural biotechnology journal · 2025Review
- Post-translational digital data encoding into the genomes of mammalian cell populations.bioRxiv : the preprint server for biology · 2024Article
31 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
DNA is an emerging medium for digital data and its adoption can be accelerated by synthesis processes specialized for storage applications. Here, we describe a de novo enzymatic synthesis strategy designed for data storage which harnesses the template-independent polymerase terminal deoxynucleotidyl transferase (TdT) in kinetically controlled conditions. Information is stored in transitions between non-identical nucleotides of DNA strands. To produce strands representing user-defined content, nucleotide substrates are added iteratively, yielding short homopolymeric extensions whose lengths are controlled by apyrase-mediated substrate degradation. With this scheme, we synthesize DNA strands carrying 144 bits, including addressing, and demonstrate retrieval with streaming nanopore sequencing. We further devise a digital codec to reduce requirements for synthesis accuracy and sequencing coverage, and experimentally show robust data retrieval from imperfectly synthesized strands. This work provides distributive enzymatic synthesis and information-theoretic approaches to advance digital information storage in DNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.