ArticleThe Journal of cell biology1987
Lateral mobility of class I histocompatibility antigens in B lymphoblastoid cell membranes: modulation by cross-linking and effect of cell density.
Article in The Journal of cell biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 35 citations in OpenAlex.
- Membrane protein dynamics and functional implications in mammalian cells.Current topics in membranes · 2013Article
- Quantification of the effect of glycocalyx condition on membrane receptor interactions using an acoustic wave sensor.European biophysics journal : EBJ · 2011Article
- Measurement of two-dimensional binding constants between cell-bound major histocompatibility complex and immobilized antibodies with an acoustic biosensor.Biophysical journal · 2008Article
- Actin and agonist MHC-peptide complex-dependent T cell receptor microclusters as scaffolds for signaling.The Journal of experimental medicine · 2005Article
- Effect of hydrodynamic interactions on the diffusion of integral membrane proteins: diffusion in plasma membranes.Biophysical journal · 1995Article
- Deoxygenation affects fluorescence photobleaching recovery measurements of red cell membrane protein lateral mobility.Biophysical journal · 1994Article
- Correctly sorted molecules of a GPI-anchored protein are clustered and immobile when they arrive at the apical surface of MDCK cells.The Journal of cell biology · 1993Article
- Differences between the lateral organization of conventional and inositol phospholipid-anchored membrane proteins. A further definition of micrometer scale membrane domains.The Journal of cell biology · 1991Article
- Translational diffusion of class II major histocompatibility complex molecules is constrained by their cytoplasmic domains.The Journal of cell biology · 1989Article
- Tumor necrosis factor and immune interferon act in concert to slow the lateral diffusion of proteins and lipids in human endothelial cell membranes.The Journal of cell biology · 1988Article
- Cytoplasmic domain affects membrane expression and function of an Ia molecule.Proceedings of the National Academy of Sciences of the United States of America · 1988Article
- Class I major histocompatibility complex proteins diffuse isotropically on immune interferon-activated endothelial cells despite anisotropic cell shape and cytoskeletal organization: application of fluorescence photobleaching recovery with an elliptical beam.Proceedings of the National Academy of Sciences of the United States of America · 1988Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We have studied the lateral mobility of class 1 major histocompatibility complex (MHC) proteins in the membranes of human Epstein-Barr virus-transformed B cells using fluorescence photobleaching recovery. Class I MHC antigens were labeled with either W6/32 monoclonal antibody or its Fab fragment directly conjugated to fluorescein isothiocyanate. The diffusion coefficient of class I antigens labeled with Fab fragments of W6/32 was identical to that of a lipid analogue, fluorescein phosphatidylethanolamine, and was 10-fold greater than that of antigens labeled with intact W6/32. Furthermore, antigens labeled with Fab fragments but not with intact W6/32 had fractional mobilities identical to that of the lipid probe. The lateral mobility of class I antigens was dependent on the time of incubation with fluorescent antibody and on the presence of antibody microaggregates. Finally, class I MHC proteins labeled with intact W6/32 but not with Fab fragments were immobilized in the membranes of most cells grown in suspension at high cell density. These results suggest that, in the unperturbed state, class I MHC antigens diffuse as rapidly as membrane lipid, i.e., without cytoskeletal constraint. Cross-linking with bivalent ligand and growth to high cell density may trigger membrane events leading to slowing and immobilization of these proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.