ArticleeLife2019
Dissociating orexin-dependent and -independent functions of orexin neurons using novel Orexin-Flp knock-in mice.
Article in eLife, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Cellular properties and axonal projections of thyrotropin-releasing hormone-expressing neurons in the mouse lateral hypothalamic area.Journal of neurophysiology · 2026Article
- Empathy and prosocial behavior powered by orexin-driven theta oscillations.Science (New York, N.Y.) · 2026Article
- Neurotensin in the extended amygdala maintains wakefulness in novel environments.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Dopamine Drives Feedforward Inhibition to Orexin Feeding System, Mediating Weight Loss Induced by Morphine Addiction.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Drugs of abuse drive neurotransmitter plasticity that alters behavior: implications for mental health.Frontiers in behavioral neuroscience · 2025Review
- The role of orexin and MCH neurons in the hypothalamus in sleep-wake regulation and learning-forgetting balance.Frontiers in neuroscience · 2025Review
- Orexin neurons play contrasting roles in itch and pain neural processing via projecting to the periaqueductal gray.Communications biology · 2024Article
- Deficiency of orexin signaling during sleep is involved in abnormal REM sleep architecture in narcolepsy.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Chronic pain recruits hypothalamic dynorphin/kappa opioid receptor signalling to promote wakefulness and vigilance.Brain : a journal of neurology · 2023Article
- Hypothalamic orexinergic neurons modulate pain and itch in an opposite way: pain relief and itch exacerbation.The journal of physiological sciences : JPS · 2022Article
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- Orexin/hypocretin system dysfunction in patients with Takotsubo syndrome: A novel pathophysiological explanation.Frontiers in cardiovascular medicine · 2022Article
- The hypothalamic link between arousal and sleep homeostasis in mice.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The Impacts of Age and Sex in a Mouse Model of Childhood Narcolepsy.Frontiers in neuroscience · 2021Article
- DLK1 Expressed in Mouse Orexin Neurons Modulates Anxio-Depressive Behavior but Not Energy Balance.Brain sciences · 2020Article
- The mammalian circadian pacemaker regulates wakefulness via CRF neurons in the paraventricular nucleus of the hypothalamus.Science advances · 2020Article
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- Article
- GABA neurons in the ventral tegmental area regulate non-rapid eye movement sleep in mice.eLife · 2019Article
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 2 countries.
Funding
Abstract
Uninterrupted arousal is important for survival during threatening situations. Activation of orexin/hypocretin neurons is implicated in sustained arousal. However, orexin neurons produce and release orexin as well as several co-transmitters including dynorphin and glutamate. To disambiguate orexin-dependent and -independent physiological functions of orexin neurons, we generated a novel Orexin-flippase (Flp) knock-in mouse line. Crossing with Flp-reporter or Cre-expressing mice showed gene expression exclusively in orexin neurons. Histological studies confirmed that orexin was knock-out in homozygous mice. Orexin neurons without orexin showed altered electrophysiological properties, as well as received decreased glutamatergic inputs. Selective chemogenetic activation revealed that both orexin and co-transmitters functioned to increase wakefulness, however, orexin was indispensable to promote sustained arousal. Surprisingly, such activation increased the total time spent in cataplexy. Taken together, orexin is essential to maintain basic membrane properties and input-output computation of orexin neurons, as well as to exert awake-sustaining aptitude of orexin neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.