ArticleGenome biology2019
Chromatin accessibility plays a key role in selective targeting of Hox proteins.
Article in Genome biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- HOXD1 regulates neural crest cells differentiation and polycerate development in sheep.Scientific reports · 2025Article
- Experimental approaches to investigate biophysical interactions between homeodomain transcription factors and DNA.Biochimica et biophysica acta. Gene regulatory mechanisms · 2025Review
- Chromatin enables precise and scalable gene regulation with factors of limited specificity.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- START domains generate paralog-specific regulons from a single network architecture.Nature communications · 2024Article
- Multimodal Hox5 activity generates motor neuron diversity.Communications biology · 2024Article
- Novel Antennapedia and Ultrabithorax trimeric complexes with TBP and Exd regulate transcription.Hereditas · 2024Article
- The evolution and mutational robustness of chromatin accessibility in Drosophila.Genome biology · 2023Article
- Floral Homeotic Factors: A Question of Specificity.Plants (Basel, Switzerland) · 2023Review
- Developmental phenomics suggests that H3K4 monomethylation confers multi-level phenotypic robustness.Cell reports · 2022Article
- Trimeric complexes of Antp-TBP with TFIIEβ or Exd modulate transcriptional activity.Hereditas · 2022Article
- Micromanagement ofJournal of developmental biology · 2022Review
- Cell-type-specific Hox regulatory strategies orchestrate tissue identity.Current biology : CB · 2021Article
- The Hox protein conundrum: The "specifics" of DNA binding for Hox proteins and their partners.Developmental biology · 2021Review
- A TALE/HOX code unlocks WNT signalling response towards paraxial mesoderm.Nature communications · 2021Article
- Machine learning predicts nucleosome binding modes of transcription factors.BMC bioinformatics · 2021Article
- Mechanisms Underlying Hox-Mediated Transcriptional Outcomes.Frontiers in cell and developmental biology · 2021Review
- HOX paralogs selectively convert binding of ubiquitous transcription factors into tissue-specific patterns of enhancer activation.PLoS genetics · 2020Article
- Differential abilities to engage inaccessible chromatin diversify vertebrate Hox binding patterns.Development (Cambridge, England) · 2020Article
- Unexpected mutual regulation underlies paralogue functional diversification and promotes epithelial tissue maturation in Tribolium.Communications biology · 2020Article
- HOX gene cluster (de)regulation in brain: from neurodevelopment to malignant glial tumours.Cellular and molecular life sciences : CMLS · 2020Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
backgroundHox transcription factors specify segmental diversity along the anterior-posterior body axis in metazoans. While the different Hox family members show clear functional specificity in vivo, they all show similar binding specificity in vitro and a satisfactory understanding of in vivo Hox target selectivity is still lacking.
resultsUsing transient transfection in Kc167 cells, we systematically analyze the binding of all eight Drosophila Hox proteins. We find that Hox proteins show considerable binding selectivity in vivo even in the absence of canonical Hox cofactors Extradenticle and Homothorax. Hox binding selectivity is strongly associated with chromatin accessibility, being highest in less accessible chromatin. Individual Hox proteins exhibit different propensities to bind less accessible chromatin, and high binding selectivity is associated with high-affinity binding regions, leading to a model where Hox proteins derive binding selectivity through affinity-based competition with nucleosomes. Extradenticle/Homothorax cofactors generally facilitate Hox binding, promoting binding to regions in less accessible chromatin but with little effect on the overall selectivity of Hox targeting. These cofactors collaborate with Hox proteins in opening chromatin, in contrast to the pioneer factor, Glial cells missing, which facilitates Hox binding by independently generating accessible chromatin regions.
conclusionsThese studies indicate that chromatin accessibility plays a key role in Hox selectivity. We propose that relative chromatin accessibility provides a basis for subtle differences in binding specificity and affinity to generate significantly different sets of in vivo genomic targets for different Hox proteins.
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