ArticleInternational journal of molecular sciences2019
Chondrogenic Differentiation of Pluripotent Stem Cells under Controllable Serum-Free Conditions.
Article in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Tracheal Regeneration: Recent Progress in the Application of Stem Cells in Tracheal Bioengineering.International journal of molecular sciences · 2026Review
- Long-term evaluation of human iPSC-derived cartilage for repairing chondral defects.NPJ Regenerative medicine · 2025Article
- Differentiation of stem cells into chondrocytes and their potential clinical application in cartilage regeneration.Histochemistry and cell biology · 2025Review
- Predictive Neuromarker Patterns for Calcification Metaplasia in Early Tendon Healing.Veterinary sciences · 2024Article
- Harnessing the dental cells derived from human induced pluripotent stem cells for hard tissue engineering.Journal of advanced research · 2024Article
- Automated xeno-free chondrogenic differentiation from human embryonic stem cells: Enhancing efficiency and ensuring high-quality mass production.Regenerative therapy · 2024Article
- Stem cell-based ischemic stroke therapy: Novel modifications and clinical challenges.Asian journal of pharmaceutical sciences · 2024Review
- Advances in cartilage tissue regeneration: a review of stem cell therapies, tissue engineering, biomaterials, and clinical trials.EXCLI journal · 2024Review
- Insights into the present and future of cartilage regeneration and joint repair.Cell regeneration (London, England) · 2022Review
- Tissue Engineering of Cartilage Using Collagen Scaffold Enriched with Plant Polysaccharides.Cartilage · 2021Article
- 3D Bioprinting of Hydrogels for Cartilage Tissue Engineering.Gels (Basel, Switzerland) · 2021Review
- Tissue Engineering for Musculoskeletal Regeneration and Disease Modeling.Handbook of experimental pharmacology · 2021Article
- Extracellular matrix-based biomaterials as adipose-derived stem cell delivery vehicles in wound healing: a comparative study between a collagen scaffold and two xenografts.Stem cell research & therapy · 2020Article
- Secretome studies of mesenchymal stromal cells (MSCs) isolated from three tissue sources reveal subtle differences in potency.In vitro cellular & developmental biology. Animal · 2020Article
- A lithium-containing biomaterial promotes chondrogenic differentiation of induced pluripotent stem cells with reducing hypertrophy.Stem cell research & therapy · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The repair of damaged articular cartilage using currently available implantation techniques is not sufficient for the full recovery of patients. Pluripotent stem cells (iPSC)-based therapies could bring new perspectives in the treatment of joint diseases. A number of protocols of in vitro differentiation of iPSC in chondrocytes for regenerative purposes have been recently described. However, in order to use these cells in clinics, the elimination of animal serum and feeder cells is essential. In our study, a strictly defined and controllable protocol was designed for the differentiation of pluripotent stem cells (BG01V, ND 41658*H, GPCCi001-A) in chondrocyte-like cells in serum- and a feeder cell-free system, using the embryoid bodies step. The extension of the protocol and culture conditions (monolayer versus 3D culture) was also tested after the initial 21 days of chondrogenic differentiation. Promotion of the chondrogenic differentiation in 3D culture via the elevated expression of genes related to chondrogenesis was achieved. Using immunofluorescence and immunohistochemistry staining techniques, the increased deposition of the specific extracellular matrix was indicated. As a result, chondrocyte-like cells in the early stages of their differentiation using pellet culture under fully controlled and defined conditions were obtained.
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Registered trials
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