Time-Gated Luminescent In Situ Hybridization (LISH): Highly Sensitive Detection of Pathogenic

Nima Sayyadi, Russell E Connally, Thomas S Lawson, Jingli Yuan, Nicolle H Packer, James A Piper

Open access · goldAbstract read

In one paragraph

Article in Molecules (Basel, Switzerland), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

0.3field-weighted citation impact, top 45% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Nima SayyadiDepartment of Molecular Sciences, Macquarie University, Sydney, NSW 2109, Australia. nima.sayyadi@mq.edu.au.ORCID 0000-0002-0044-2170

Russell E ConnallyDepartment of Physics and Astronomy, Macquarie University, Sydney, NSW 2109, Australia. russell.connally@mq.edu.au.

Thomas S LawsonARC Centre of Excellence for Nanoscale Biophotonics (CNBP), Macquarie University, Sydney, NSW 2109, Australia. thomas.lawson@mq.edu.au.

Jingli YuanState Key Laboratory of Fine Chemicals, School of Chemistry, Dalian University of Technology, Dalian 116023, China. jlyuan@dlut.edu.cn.

Nicolle H PackerDepartment of Molecular Sciences, Macquarie University, Sydney, NSW 2109, Australia. nicki.packer@mq.edu.au.

James A PiperARC Centre of Excellence for Nanoscale Biophotonics (CNBP), Macquarie University, Sydney, NSW 2109, Australia. jim.piper@mq.edu.au.

Macquarie University · AUDalian University of Technology · CN

Funding

ARC Centre of Excellence for Nanoscale BioPhotonics Grant CE140100003the Paige Weatherspoon Foundation, the Macquarie University IP Development Funding Grant 9201002101

6 · The paper itself

Abstract

We describe simple direct conjugation of a single TEGylated Europium chelate to DNA that binds to intracellular rRNA and is then detected using a homogeneous luminescent in situ hybridisation (LISH) technique. As a proof-of-principle,

Indexed as

In Situ HybridizationLuminescenceLuminescent MeasurementsChromatography, High Pressure LiquidHumansRNA, Ribosomal, 16SStaphylococcal InfectionsStaphylococcus aureusRNA, Ribosomal, 16Shomogeneous luminescent in situ hybridisationStaphylococcus aureus (S. aureus) detectionTEGylated Europium chelatetime-gated luminescent microscopy

Identifiers

PMID31159269

DOI10.3390/molecules24112083

PMCPMC6600140

OpenAlexW2948006732

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.