Evidence map›Paper›PMID 31158477›Full record

ArticleNeuroImage2019

QUEST MRI assessment of fetal brain oxidative stress in utero.

Bruce A Berkowitz, Roberto Romero, Robert H Podolsky, Karen M Lins-Childers, Yimin Shen, Tilman Rosales, Youssef Zaim Wadghiri, D Minh Hoang, Marcia Arenas-Hernandez, Valeria Garcia-Flores and 3 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in NeuroImage, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Neonatal Anesthesia and Oxidative Stress.Antioxidants (Basel, Switzerland) · 2022
    Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 6 institutions in 1 country.

Bruce A BerkowitzDepartment of Ophthalmology, Visual and Anatomical Sciences, Wayne State University School of Medicine, Detroit, Michigan, 48201, USA. Electronic address: baberko@med.wayne.edu.
Roberto RomeroPerinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, U.S. Department of Health and Human Services (NICHD/NIH/DHHS), Bethesda, Maryland, 20847, and Detroit, Michigan, 48201, USA; Department of Obstetrics and Gynecology, University of Michigan Health System, Ann Arbor, Michigan, 48109, USA; Department of Epidemiology and Biostatistics, College of Human Medicine, Michigan State University, East Lansing, Michigan, 48824, USA; Center for Molecular Medicine and Genetics, Wayne State University, Detroit, Michigan, 48201, USA; Detroit Medical Center, Detroit, Michigan, 48201, USA.
Robert H PodolskyBeaumont Research Institute, Beaumont Health, Royal Oak, Michigan, 48073, USA.
Karen M Lins-ChildersBeaumont Research Institute, Beaumont Health, Royal Oak, Michigan, 48073, USA.
Yimin ShenDepartment of Radiology, Wayne State University School of Medicine, Detroit, Michigan, 48201, USA.
Tilman RosalesDepartment of Ophthalmology, Visual and Anatomical Sciences, Wayne State University School of Medicine, Detroit, Michigan, 48201, USA.
Youssef Zaim WadghiriDepartment of Radiology, Center for Advanced Imaging Innovation and Research (CAI2R), Bernard and Irene Schwartz Center for Biomedical Imaging, NYU School of Medicine and NYU Langone Health, New York, New York, 10016, USA.
D Minh HoangDepartment of Radiology, Center for Advanced Imaging Innovation and Research (CAI2R), Bernard and Irene Schwartz Center for Biomedical Imaging, NYU School of Medicine and NYU Langone Health, New York, New York, 10016, USA.
Marcia Arenas-HernandezPerinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, U.S. Department of Health and Human Services (NICHD/NIH/DHHS), Bethesda, Maryland, 20847, and Detroit, Michigan, 48201, USA; Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, Michigan, 48201, USA.
Valeria Garcia-FloresPerinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, U.S. Department of Health and Human Services (NICHD/NIH/DHHS), Bethesda, Maryland, 20847, and Detroit, Michigan, 48201, USA; Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, Michigan, 48201, USA.
George SchwenkelPerinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, U.S. Department of Health and Human Services (NICHD/NIH/DHHS), Bethesda, Maryland, 20847, and Detroit, Michigan, 48201, USA; Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, Michigan, 48201, USA.
Bogdan PanaitescuPerinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, U.S. Department of Health and Human Services (NICHD/NIH/DHHS), Bethesda, Maryland, 20847, and Detroit, Michigan, 48201, USA; Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, Michigan, 48201, USA.
Nardhy Gomez-LopezPerinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, U.S. Department of Health and Human Services (NICHD/NIH/DHHS), Bethesda, Maryland, 20847, and Detroit, Michigan, 48201, USA; Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, Michigan, 48201, USA; Department of Biochemistry, Microbiology and Immunology, Wayne State University School of Medicine, Detroit, Michigan, 48201, USA. Electronic address: nardhy.gomez-lopez@wayne.edu.
Wayne State University · USAdvanced Imaging Research (United States) · USBeaumont Health · USEunice Kennedy Shriver National Institute of Child Health and Human Development · USNational Institutes of Health · USUnited States Department of Health and Human Services · US

Funding

Prenatal Diagnosis Of Congenital AnomaliesZIAHD002401 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI ROMERO, ROBERTO · 2009 to 2025
$81.1M
TR&D 4: Revealing Microstructure: Biophysical modeling and validation for discovery and clinical careP41EB017183 · NIBIB · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · PI Christopher M Collins · 2014 to 2026
$19.3M
Novel Imaging Biomarker for Treating Spatial Memory Loss in Prodromal Alzheimer's Disease ModelsR01AG058171 · NIA · WAYNE STATE UNIVERSITY · PI BENNETT, BRIAN M, BERKOWITZ, BRUCE A. · 2017 to 2021
$2.8M
Novel Imaging of rod oxidative stress in retinal disease and treatment in vivoR01EY026584 · NEI · WAYNE STATE UNIVERSITY · PI BERKOWITZ, BRUCE A. · 2016 to 2019
$1.5M
NEI NIH HHS R01 EY026584NIA NIH HHS R01 AG058171NIBIB NIH HHS P41 EB017183NICHD NIH HHS HHSN275201300006C
6 · The paper itself

Abstract

purposeTo achieve sufficient precision of R1 (=1/T1) maps of the fetal brain in utero to perform QUEnch-assiSTed (QUEST) MRI in which a significant anti-oxidant-induced reduction in R1 indicates oxidative stress.

methodsC57BL/6 mouse fetuses in utero were gently and non-surgically isolated and secured using a homemade 3D printed clip. Using a commercial receive-only surface coil, brain maps of R1, an index sensitive to excessive and continuous free radical production, were collected using either a conventional Cartesian or a non-Cartesian (periodically rotated overlapping parallel lines with enhanced reconstruction) progressive saturation sequence. Data were normalized to the shortest TR time to remove bias. To assess oxidative stress, brain R1 maps were acquired on the lipopolysaccharide (LPS) model of preterm birth ± rosiglitazone (ROSI, which has anti-oxidant properties); phosphate buffered saline (PBS) controls ± ROSI were similarly studied.

resultsSufficient quality R1 maps were generated by a combination of the 3D printed clip, surface coil detection, non-Cartesian sequence, and normalization scheme ensuring minimal fetal movement, good detection sensitivity, reduced motion artifacts, and minimal baseline variations, respectively. In the LPS group, the combined caudate-putamen and thalamus region R1 was reduced (p < 0.05) with ROSI treatment consistent with brain oxidative stress; no evidence for oxidative stress was found in the pons region. In the PBS control group, brain R1's did not change with ROSI treatment.

conclusionThe sensitivity and reproducibility of the combined approaches described herein enabled first-time demonstration of regional oxidative stress measurements of the fetal brain in utero using QUEST MRI.

Indexed as

Oxidative StressAnimalsBrainDisease Models, AnimalEmbryo, MammalianFemaleMagnetic Resonance ImagingMiceMice, Inbred C57BLPregnancyPrenatal DiagnosisEmbryoFree radicalsQuench-assisted MRIReactive oxygen species

Identifiers

PMID31158477
PMCPMC6703912
OpenAlexW2947127910

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.