ArticlePLoS neglected tropical diseases2019
Field diagnosis and genotyping of chikungunya virus using a dried reverse transcription loop-mediated isothermal amplification (LAMP) assay and MinION sequencing.
Article in PLoS neglected tropical diseases, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- A rapid multiplex platform for simultaneous detection of chikungunya virus, dengue virus, and dengue serotyping based on isothermal amplification and lateral flow dipsticks.Infectious diseases of poverty · 2026Article
- The Application of Reverse Transcription Loop-mediated Isothermal Amplification to Detect Cell-Free Seminal mRNA.Molecular biotechnology · 2026Article
- Development and validation of RT-LAMP for detecting yellow fever virus in non-human primates samples from Brazil.Scientific reports · 2024Article
- Rapid and sensitive detection of chikungunya virus using one-tube, reverse transcription, semi-nested multi-enzyme isothermal rapid amplification, and lateral flow dipstick assays.Journal of clinical microbiology · 2024Article
- Schistosomiasis diagnosis: Challenges and opportunities for elimination.PLoS neglected tropical diseases · 2024Review
- Multiplexing LAMP Assays: A Methodological Review and Diagnostic Application.International journal of molecular sciences · 2024Review
- Development and field validation of a reverse transcription loop-mediated isothermal amplification assay (RT-LAMP) for the rapid detection of chikungunya virus in patient and mosquito samples.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2024Article
- Monkeypox.Lancet (London, England) · 2023Review
- Article
- Comparison ofFrontiers in microbiology · 2023Article
- Understanding the Biology and Immune Pathogenesis of Chikungunya Virus Infection for Diagnostic and Vaccine Development.Viruses · 2022Review
- Recent molecular techniques for the diagnosis of Zika and Chikungunya infections: A systematic review.Heliyon · 2022Article
- Molecular Characterization of Increased Amplicon Lengths in SARS-CoV-2 Reverse Transcription Loop-Mediated Isothermal Amplification Assays.Journal of biomolecular techniques : JBT · 2021Article
- Diagnosing point-of-care diagnostics for neglected tropical diseases.PLoS neglected tropical diseases · 2021Review
- Development of a Multiplex Loop-Mediated Isothermal Amplification (LAMP) Method for Simultaneous Detection of Spotted Fever Group Rickettsiae and Malaria Parasites by Dipstick DNA Chromatography.Diagnostics (Basel, Switzerland) · 2020Article
- Development of a bio-inkjet printed LAMP test kit for detecting human African trypanosomiasis.PLoS neglected tropical diseases · 2020Article
- Portable sequencer in the fight against infectious disease.Journal of human genetics · 2020Review
- A simple method for sequencing the whole human mitochondrial genome directly from samples and its application to genetic testing.Scientific reports · 2019Article
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Authors and funding
16 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Detection and sequencing of chikungunya virus (CHIKV) genome was performed using a combination of a modified reverse transcription loop-mediated isothermal amplification (RT-LAMP) method and a MinION sequencer. We developed the protocol for drying all the reagents for the RT-LAMP in a single reaction tube. Using this system, the CHIKV genome was effectively amplified under isothermal conditions, and used as a template for MinION sequencing with a laptop computer. Our in-house RT-LAMP method and MinION sequencing system were also validated with RNAs and serum samples from recent outbreaks of CHIKV patients in Brazil. The obtained sequence data confirmed the CHIKV outbreaks and identified the genotype. In summary, our established inexpensive on-site genome detection and sequencing system is applicable for both diagnosis of CHIKV infected patients and genotyping of the CHIKV virus in future outbreak in remote areas.
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