ArticleGigaScience2019
A chromosome-scale assembly of the major African malaria vector Anopheles funestus.
Article in GigaScience, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Chromosomal inversions modulate 3D chromatin interactions and regulatory architecture inResearch square · 2026Article
- Phylogenetic taxonomy of the Zambian Anopheles coustani group using a mitogenomics approach.Malaria journal · 2025Article
- Association of a rapidly selected 4.3kb transposon-containing structural variation with a P450-based resistance to pyrethroids in the African malaria vector Anopheles funestus.PLoS genetics · 2024Article
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- Advances in the genetic characterization of the malaria vector, Anopheles funestus, and implications for improved surveillance and control.Malaria journal · 2023Review
- Standing genetic variation and chromosome differences drove rapid ecotype formation in a major malaria mosquito.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The duplicated P450s CYP6P9a/b drive carbamates and pyrethroids cross-resistance in the major African malaria vector Anopheles funestus.PLoS genetics · 2023Article
- The Invasive Mosquitoes of Canada: An Entomological, Medical, and Veterinary Review.The American journal of tropical medicine and hygiene · 2022Review
- Multi-omics analysis identifies a CYP9K1 haplotype conferring pyrethroid resistance in the malaria vector Anopheles funestus in East Africa.Molecular ecology · 2022Article
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- Whole Genome Sequencing Contributions and Challenges in Disease Reduction Focused on Malaria.Biology · 2022Review
- Anopheles mosquitoes reveal new principles of 3D genome organization in insects.Nature communications · 2022Article
- The ecdysone receptor regulates several key physiological factors in Anopheles funestus.Malaria journal · 2022Article
- Advances and opportunities in malaria population genomics.Nature reviews. Genetics · 2021Review
- Chromosome-level genome assemblies of the malaria vectors Anopheles coluzzii and Anopheles arabiensis.GigaScience · 2021Article
- Hidden genomic features of an invasive malaria vector, Anopheles stephensi, revealed by a chromosome-level genome assembly.BMC biology · 2021Article
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- HapSolo: an optimization approach for removing secondary haplotigs during diploid genome assembly and scaffolding.BMC bioinformatics · 2021Article
- Radiation with reticulation marks the origin of a major malaria vector.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
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Authors and funding
7 authors.
Funding
Abstract
backgroundAnopheles funestus is one of the 3 most consequential and widespread vectors of human malaria in tropical Africa. However, the lack of a high-quality reference genome has hindered the association of phenotypic traits with their genetic basis in this important mosquito.
findingsHere we present a new high-quality A. funestus reference genome (AfunF3) assembled using 240× coverage of long-read single-molecule sequencing for contigging, combined with 100× coverage of short-read Hi-C data for chromosome scaffolding. The assembled contigs total 446 Mbp of sequence and contain substantial duplication due to alternative alleles present in the sequenced pool of mosquitos from the FUMOZ colony. Using alignment and depth-of-coverage information, these contigs were deduplicated to a 211 Mbp primary assembly, which is closer to the expected haploid genome size of 250 Mbp. This primary assembly consists of 1,053 contigs organized into 3 chromosome-scale scaffolds with an N50 contig size of 632 kbp and an N50 scaffold size of 93.811 Mbp, representing a 100-fold improvement in continuity versus the current reference assembly, AfunF1.
conclusionThis highly contiguous and complete A. funestus reference genome assembly will serve as an improved basis for future studies of genomic variation and organization in this important disease vector.
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