Evidence map›Paper›PMID 31156296›Full record

ArticleTrends in cell & molecular biology2018

Spontaneous and cytokine-induced hole formation in epithelial cell layers: Implications for barrier function studies with the gingival cell culture, Gie-3B11, and other epithelial models.

Elizabeth Rybakovsky, Nicole B Buleza, Kevt'her Hoxha, Katherine M DiGuilio, Elizabeth S McCluskey, Cary L Friday, Patrick J Callaghan, Daniil V Moskalenko, Biao Zuo, Sunil Thomas and 1 more

Abstract read
PubMed Full text at PMC
In one paragraph

Article in Trends in cell & molecular biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Elizabeth RybakovskyLankenau Institute for Medical Research, 100 Lancaster Avenue, Wynnewood, PA 19096.
Nicole B BulezaDepartment of Biology, Drexel University, Philadelphia, PA, 19104.
Kevt'her HoxhaDepartment of Biology, Drexel University, Philadelphia, PA, 19104.
Katherine M DiGuilioLankenau Institute for Medical Research, 100 Lancaster Avenue, Wynnewood, PA 19096.
Elizabeth S McCluskeyDepartment of Biology, Drexel University, Philadelphia, PA, 19104.
Cary L FridayLankenau Institute for Medical Research, 100 Lancaster Avenue, Wynnewood, PA 19096.
Patrick J CallaghanLankenau Institute for Medical Research, 100 Lancaster Avenue, Wynnewood, PA 19096.
Daniil V MoskalenkoDepartment of Biology, Holy Family University, Philadelphia, PA, 19114.
Biao ZuoElectron Microscopy Resource Laboratory, Department of Biochemistry & Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Sunil ThomasLankenau Institute for Medical Research, 100 Lancaster Avenue, Wynnewood, PA 19096.
James M MullinLankenau Institute for Medical Research, 100 Lancaster Avenue, Wynnewood, PA 19096.

Funding

Ebola Virus VP40-Host Interactions In VIvoR21AI059277 · NIAID · UNIVERSITY OF PENNSYLVANIA · PI HARTY, RONALD N. · 2004 to 2005
$620k
Dueling PPxY Motifs of Filovirus VP40 and Host Angiomotin: Effects on Innate Immune Defenses and Tight Junction Integrity at Immune Privileged SitesR21AI139392 · NIAID · UNIVERSITY OF PENNSYLVANIA · PI HARTY, RONALD N · 2018 to 2019
$462k
NIAID NIH HHS R21 AI059277NIAID NIH HHS R21 AI139392
6 · The paper itself

Abstract

Epithelial barrier function studies often attribute alterations in barrier function to induced changes in tight junctional (TJ) complexes. The occurrence of spontaneous and cytokine-induced, focal cell detachment in cell layers of the human gingival epithelial cell line, Gie-3B11, highlights the danger of this assumption without confirmatory experimentation. Gie-3B11 cell layers manifest morphological polarity, TJ complexes and barrier function after confluence but fail to then maintain a stable epithelial barrier. Transepithelial electrical resistance rises to over 100 ohms x cm

Indexed as

cell deathcytokineepithelial barrier functiongingivaltight junction

Identifiers

PMID31156296
PMCPMC6542284

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.