Evidence map›Paper›PMID 31155061›Full record

ArticleMethods in enzymology2019

Small molecule target identification using photo-affinity chromatography.

Seung-Yong Seo, Timothy W Corson

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in enzymology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Review
  2. Advances in Anthelmintic Target Identification.International journal of molecular sciences · 2025
    Review
  3. Review
  4. Review
  5. Review
  6. Sensing of Antibiotic-Bacteria Interactions.Antibiotics (Basel, Switzerland) · 2023
    Review
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Seung-Yong SeoCollege of Pharmacy, Gachon University, Incheon, South Korea.
Timothy W CorsonIndiana University School of Medicine, Indianapolis, IN, United States. Electronic address: tcorson@iu.edu.

Funding

Ferrochelatase as a mediator of ocular angiogenesisR01EY025641 · NEI · UNIVERSITY OF TORONTO · PI Timothy W Corson · 2016 to 2026
$3.5M
NEI NIH HHS R01 EY025641
6 · The paper itself

Abstract

Identification of the protein targets of bioactive small molecules is a routine challenge in chemical biology and phenotype-based drug discovery. Recent years have seen an explosion of approaches to meeting this challenge, but the traditional method of affinity pulldowns remains a practical choice in many contexts. This technique can be used as long as an affinity probe can be synthesized, usually with a crosslinking moiety to enable photo-affinity pulldowns. It can be applied to varied tissue types and can be performed with minimal specialized equipment. Here, we provide our protocol for photo-affinity pulldown experiments, with notes on making this method generally applicable to varied target identification challenges.

Indexed as

AnimalsChromatography, AffinityClick ChemistryDrug DiscoveryHumansPhotochemical ProcessesProteinsProteomicsSmall Molecule LibrariesProteinsSmall Molecule LibrariesBiotinClick chemistryInhibitorMass spectrometryNatural productOff-targetPhotoreactive groupSmall moleculeSynthetic chemistryTarget identification

Identifiers

PMID31155061
PMCPMC6729133

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.