ArticleMolecular biology reports2019
Addressing the impact of different fetal bovine serum percentages on mesenchymal stem cells biological performance.
Article in Molecular biology reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- BioMarrow: An accessible and reproducible 3D patient-derived bone marrow model for advancing research and clinical applications.Translational oncology · 2026Article
- Optimization of anBiology methods & protocols · 2026Article
- Passage- and serum-dependent changes of adipose-derived stromal cellsInnovative surgical sciences · 2025Article
- IMDM-20 enhances neutrophilic features during 1.3 % DMSO-mediated differentiation of HL-60 cells.Biochemistry and biophysics reports · 2025Article
- Comparative Proteomic Characterization of Serum-Free Cultivated HepG2 Cells Reveals Upregulated Drug Metabolism and Increased Oxidative Stress Protection.Journal of proteome research · 2025Article
- Combining dopamine and vitamin E enhances the differentiation to cholinergic neurons of mesenchymal stem cells.Journal of neuropathology and experimental neurology · 2025Article
- Article
- Hypoxic Stress Induces Complement-Mediated Lysis of Mesenchymal Stem Cells by Downregulating Factor H and CD59.Tissue engineering and regenerative medicine · 2025Article
- Ultrastructural Visualization of the Nuclear Envelope in HeLa Cells.Methods in molecular biology (Clifton, N.J.) · 2025Article
- In vitro assessment of emerging mycotoxins co-occurring in cheese: a potential health hazard.Archives of toxicology · 2024Article
- Effects of high glucose and severe hypoxia on the biological behavior of mesenchymal stem cells at various passages.World journal of stem cells · 2024Article
- The Key Role of Liraglutide in Preventing Autophagy of Vascular Smooth Muscle Cells in High Glucose ConditionsBalkan medical journal · 2024Article
- Increasing sustainability and reproducibility ofFrontiers in toxicology · 2024Article
- Human mesenchymal stem cells exhibit altered mitochondrial dynamics and poor survival in high glucose microenvironment.World journal of stem cells · 2023Article
- Extracellular Vesicle Depletion Protocols of Foetal Bovine Serum Influence Umbilical Cord Mesenchymal Stromal Cell Phenotype, Immunomodulation, and Particle Release.International journal of molecular sciences · 2023Article
- Notch and Wnt Signaling Modulation to Enhance DPSC Stemness and Therapeutic Potential.International journal of molecular sciences · 2023Review
- Molecular and epigenetic alterations in normal and malignant myelopoiesis in human leukemia 60 (HL60) promyelocytic cell line model.Frontiers in cell and developmental biology · 2023Article
- The dark side of foetal bovine serum in extracellular vesicle studies.Journal of extracellular vesicles · 2022Review
- Design of a flexing organ-chip to modelBiomicrofluidics · 2022Article
- Characterisation of Extracellular Vesicles from Equine Mesenchymal Stem Cells.International journal of molecular sciences · 2022Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human mesenchymal stem cells (MSCs) are presently on the top of hierarchy in the field of stem cell therapy, due to their miraculous therapeutic abilities in diminishing the symptoms of many chronic diseases and initiating regeneration and repair for various damaged tissues and organs. The foremost initial step to reach high success rate in any MSCs based study is the optimization of culture growth media by establishing a suitable fetal bovine serum (FBS) percentage that suits the purpose of MSCs based experiment. Choosing the suitable FBS percentage is a controversial issue and merely depends on the researchers experience and suggested recommendations by the suppliers. Despite the huge improvements in overall MSCs investigating approaches, there are no definite protocols that set up a range of FBS percentages that can be followed. Toward achieving this objective, we evaluate in the present report the effect of using various FBS percentages (5-20%) added to DMEM low glucose media, on the biological behaviour of MSCs. Growing MSCs in high FBS percentages containing culture media (15% and 20% FBS) increase the proliferation and expansion rate of MSCs, although it decreases the immunosuppressive properties. On the other hand, adding low FBS percentage (7% FBS) to MSCs culture media enhanced the immunosuppression characteristics of MSCs, even though the proliferation rate was moderately reduced. 7% FBS is the cut off percentage that can be used without negatively altering major MSCs biological properties in which using 5% FBS will cause a tremendous decrease in the proliferation capacity and immunosuppressive properties. This report may assist other researchers in choosing appropriate FBS percentage when preparing MSCs culture media that serve the purpose of their MSCs based studies.
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