ArticleMethods (San Diego, Calif.)2020
Imaging tripartite synapses using super-resolution microscopy.
Article in Methods (San Diego, Calif.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 35 citations in OpenAlex.
- Article
- Recent optical approaches for anatomical and functional dissection of neuron-astrocyte circuitry.The Journal of physiology · 2026Review
- Astrocytes of the hippocampus and responses to periprandial neuroendocrine hormones.Physiology & behavior · 2025Review
- Advancements in Single-Molecule Fluorescence Detection Techniques and Their Expansive Applications in Drug Discovery and Neuroscience.Biosensors · 2025Review
- Astrocyte-derived MFG-E8 facilitates microglial synapse elimination in Alzheimer's disease mouse models.bioRxiv : the preprint server for biology · 2024Article
- Current evidence of synaptic dysfunction after stroke: Cellular and molecular mechanisms.CNS neuroscience & therapeutics · 2024Review
- GLT-1a glutamate transporter nanocluster localization is associated with astrocytic actin and neuronal Kv2 clusters at sites of neuron-astrocyte contact.Frontiers in cell and developmental biology · 2024Article
- Neurexin-3 subsynaptic densities are spatially distinct from Neurexin-1 and essential for excitatory synapse nanoscale organization in the hippocampus.Nature communications · 2023Article
- Human neutrophils communicate remotely via calcium-dependent glutamate-induced glutamate release.iScience · 2023Article
- Tracking the Message: Applying Single Molecule Localization Microscopy to Cellular RNA Imaging.Chembiochem : a European journal of chemical biology · 2023Review
- STORM Super-Resolution Imaging of CBMethods in molecular biology (Clifton, N.J.) · 2023Article
- Reinforcing Interdisciplinary Collaborations to Unravel the Astrocyte "Calcium Code".Journal of molecular neuroscience : MN · 2022Review
- Estimating the glutamate transporter surface density in distinct sub-cellular compartments of mouse hippocampal astrocytes.PLoS computational biology · 2022Article
- Revealing the contribution of astrocytes to glutamatergic neuronal transmission.Frontiers in cellular neuroscience · 2022Review
- The Specific Role of Reactive Astrocytes in Stroke.Frontiers in cellular neuroscience · 2022Review
- Super-resolution imaging to reveal the nanostructure of tripartite synapses.Neuronal signaling · 2021Review
- Differential Proximity of Perisynaptic Astrocytic Best1 at the Excitatory and Inhibitory Tripartite Synapses in APP/PS1 and MAOB-KO Mice Revealed by Lattice Structured Illumination Microscopy.Experimental neurobiology · 2021Article
- Robust adaptive optics for localization microscopy deep in complex tissue.Nature communications · 2021Article
- Article
- Advanced imaging and labelling methods to decipher brain cell organization and function.Nature reviews. Neuroscience · 2021Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Astroglia are vital facilitators of brain development, homeostasis, and metabolic support. In addition, they are also essential to the formation and regulation of synaptic circuits. Due to the extraordinary complex, nanoscopic morphology of astrocytes, the underlying cellular mechanisms have been poorly understood. In particular, fine astrocytic processes that can be found in the vicinity of synapses have been difficult to study using traditional imaging techniques. Here, we describe a 3D three-colour super-resolution microscopy approach to unravel the nanostructure of tripartite synapses. The method is based on the SMLM technique direct stochastic optical reconstruction microscopy (dSTORM) which uses conventional fluorophore-labelled antibodies. This approach enables reconstructing the nanoscale localisation of individual astrocytic glutamate transporter (GLT-1) molecules surrounding presynaptic (bassoon) and postsynaptic (Homer1) protein localisations in fixed mouse brain sections. However, the technique is readily adaptable to other types of targets and tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.