ArticleDevelopmental biology2019
Blood feeding activates the vitellogenic stage of oogenesis in the mosquito Aedes aegypti through inhibition of glycogen synthase kinase 3 by the insulin and TOR pathways.
Article in Developmental biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers, 1 of them a synthesis that pooled it.
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Who cites it
50 citing papers in PubMed, 1 synthesis or guideline pooled it, 88 citations in OpenAlex.
- The food of life: which nectar do mosquitoes feed on?-An evidence-based meta-analysis.Environmental entomology · 2025Pooled it
- Sexually Dimorphic Visual Processing in the Yellow-Fever Mosquito,bioRxiv : the preprint server for biology · 2026Article
- Knockdown of the adipokinetic hormone receptor inhibits the reproduction of female Culex pipiens pallens (Diptera: Culicidae) by downregulating L-homoserine and serotonin levels.Insect science · 2026Article
- Credit to the Fruit Fly: How the Tiny Insect Lights Up Our Understanding of Human Disease.Insects · 2026Review
- A signaling hub in the mosquito rectum coordinates reproductive investment after blood feeding.Current biology : CB · 2026Article
- Vector venom: venomics of Aedes albopictus reveals a large enzyme repertoire and novel cecropins with activity against E. coli.npj drug discovery · 2026Article
- Mutualism in disguise: A mosquito parasite with mixed transmission mode displays mutualistic traits promoting oogenesis.PLoS pathogens · 2026Article
- Population differences in reproductive resource allocation and heterosis in the invasive vector Aedes albopictus.Parasites & vectors · 2026Article
- TheFrontiers in cellular and infection microbiology · 2026Article
- Establishing and sustaining mosquito colonies: insights into morphology, bionomics, and advances in the rearing ofFrontiers in insect science · 2026Review
- Changes in the Anopheles arabiensis transcriptome and gut microbiota profiles associated with the endosymbiotic Microsporidia MB.BMC genomics · 2025Article
- Juvenile hormone induces phosphorylation of insulin/insulin-like growth factor signaling proteins in previtellogenic Aedes aegypti mosquitoes.Insect science · 2025Article
- Genomic and insulin-mediated control of metabolic homeostasis by the mosquito ecdysone-induced gene E93.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Impact of pyriproxyfen on the reproduction of pyrethroid-resistant Anopheles gambiae s.l. populations in benin: what implications for vector control?BMC infectious diseases · 2025Article
- Insulin-like peptides activate egg formation in the Asian malaria mosquito Anopheles stephensi.Parasites & vectors · 2025Article
- From Lab to Clinic and Farm: LeveragingBiology · 2025Review
- A Signaling Hub in the Mosquito Rectum Coordinates Reproductive Investment After Blood Feeding.bioRxiv : the preprint server for biology · 2025Article
- Single-cell transcriptional landscapes of Aedes aegypti midgut and fat body after a bloodmeal.Cell genomics · 2025Article
- Fraxinus foliage: does host species during adult maturation feeding alter the fecundity of emerald ash borers, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae)?Environmental entomology · 2025Article
- Ovarian transcriptome analyses indicate that weak juvenile hormone signaling underlies the molecular basis of oogenesis deficiencies in mosquitoes.BMC biology · 2025Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Most mosquitoes, including Aedes aegypti, only produce eggs after blood feeding on a vertebrate host. Oogenesis in A. aegypti consists of a pre-vitellogenic stage before blood feeding and a vitellogenic stage after blood feeding. Primary egg chambers remain developmentally arrested during the pre-vitellogenic stage but complete oogenesis to form mature eggs during the vitellogenic stage. In contrast, the signaling factors that maintain primary egg chambers in pre-vitellogenic arrest or that activate vitellogenic growth are largely unclear. Prior studies showed that A. aegypti females release insulin-like peptide 3 (ILP3) and ovary ecdysteroidogenic hormone (OEH) from brain neurosecretory cells after blood feeding. Here, we report that primary egg chambers exit pre-vitellogenic arrest by 8 h post-blood meal as evidenced by proliferation of follicle cells, endoreplication of nurse cells, and formation of cytoophidia. Ex vivo assays showed that ILP3 and OEH stimulate primary egg chambers to exit pre-vitellogenic arrest in the presence of nutrients but not in their absence. Characterization of associated pathways indicated that activation of insulin/insulin growth factor signaling (IIS) by ILP3 or OEH inactivated glycogen synthase kinase 3 (GSK3) via phosphorylation by phosphorylated Akt. GSK3 inactivation correlated with accumulation of the basic helix-loop-helix transcription factor Max and primary egg chambers exiting pre-vitellogenic arrest. Direct inhibition of GSK3 by CHIR-99021 also stimulated Myc/Max accumulation and primary egg chambers exiting pre-vitellogenic arrest. Collectively, our results identify GSK3 as a key factor in regulating the pre- and vitellogenic stages of oogenesis in A. aegypti.
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