ArticleJournal of structural biology2019
Crystal structure of F95Q epi-isozizaene synthase, an engineered sesquiterpene cyclase that generates biofuel precursors β- and γ-curcumene.
Article in Journal of structural biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Structure-Based Engineering of a Sesquiterpene Cyclase to Generate an Alcohol Product: Conversion ofBiochemistry · 2024Article
- How a 10-ACS catalysis · 2022Article
- Taxonomic Insights and Its Type Cyclization Correlation of Volatile Sesquiterpenes inMolecules (Basel, Switzerland) · 2021Review
- Mining methods and typical structural mechanisms of terpene cyclases.Bioresources and bioprocessing · 2021Review
- Structure, catalysis, and inhibition mechanism of prenyltransferase.IUBMB life · 2021Review
- An Aromatic Cluster in the Active Site ofBiochemistry · 2020Article
- Synthetic biology, combinatorial biosynthesis, and chemo‑enzymatic synthesis of isoprenoids.Journal of industrial microbiology & biotechnology · 2020Review
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Authors and funding
3 authors.
Funding
Abstract
The saturated hydrocarbon bisabolane is a diesel fuel substitute that can be derived from sesquiterpene precursors bisabolene or curcumene. These sesquiterpenes are generated from farnesyl diphosphate in reactions catalyzed by eponymous terpenoid cyclases, but they can also be generated by engineered terpenoid cyclases in which cyclization cascades have been reprogrammed by mutagenesis. Here, we describe the X-ray crystal structure determination of F95Q epi-isozizaene synthase (EIZS), in which the new activity of curcumene biosynthesis has been introduced and the native activity of epi-isozizaene biosynthesis has been suppressed. F95Q EIZS generates β- and γ-curcumene regioisomers with greater than 50% yield. Structural analysis of the closed active site conformation, stabilized by the binding of 3 Mg
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