ArticleAMB Express2019
Heterologous rhamnolipid biosynthesis by P. putida KT2440 on bio-oil derived small organic acids and fractions.
Article in AMB Express, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- Enhancing acetate-based biosurfactant production by Pseudomonas putida KT2440 through fermentation optimization.Microbial cell factories · 2026Article
- Synthetic consortia towards photosynthetically derived acetate for heterotrophic production of 1-butanol.Applied microbiology and biotechnology · 2026Article
- Engineering growth independent production: programmable control loops for high-titer rhamnolipids for improved green surfactant sustainability.Bioprocess and biosystems engineering · 2026Review
- Acetate as alternative carbon source for production of mono- and di-rhamnolipids in Pseudomonas putida KT2440.Microbial cell factories · 2026Article
- Heterologous rhamnolipid biosynthesis byApplied and environmental microbiology · 2025Article
- Two-stage conversion of syngas and pyrolysis aqueous condensate into L-malate.Biotechnology for biofuels and bioproducts · 2024Article
- Recombinant Production ofMicroorganisms · 2024Article
- Bioupgrading of the aqueous phase of pyrolysis oil from lignocellulosic biomass: a platform for renewable chemicals and fuels from the whole fraction of biomass.Bioresources and bioprocessing · 2023Review
- Leveraging substrate flexibility and product selectivity of acetogens in two-stage systems for chemical production.Microbial biotechnology · 2023Review
- High-quality physiology ofFrontiers in bioengineering and biotechnology · 2023Article
- Tuning a high performing multiplexed-CRISPRiMetabolic engineering communications · 2022Article
- Exploiting RNA thermometer-driven molecular bioprocess control as a concept for heterologous rhamnolipid production.Scientific reports · 2021Article
- Burkholderia thailandensis E264 as a promising safe rhamnolipids' producer towards a sustainable valorization of grape marcs and olive mill pomace.Applied microbiology and biotechnology · 2021Article
- Coupling an ElectroactiveMicroorganisms · 2020Article
- Integration of Genetic and Process Engineering for Optimized Rhamnolipid Production UsingFrontiers in bioengineering and biotechnology · 2020Article
- Killing Two Birds With One Stone - Strain Engineering Facilitates the Development of a Unique Rhamnolipid Production Process.Frontiers in bioengineering and biotechnology · 2020Article
- Heterologous Rhamnolipid Biosynthesis: Advantages, Challenges, and the Opportunity to Produce Tailor-Made Rhamnolipids.Frontiers in bioengineering and biotechnology · 2020Review
- Characterization of Extracellular Biosurfactants Expressed by a Pseudomonas putida Strain Isolated from the Interior of Healthy Roots from Sida hermaphrodita Grown in a Heavy Metal Contaminated Soil.Current microbiology · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
In many cases in industrial biotechnology, substrate costs make up a major part of the overall production costs. One strategy to achieve more cost-efficient processes in general is to exploit cheaper sources of substrate. Small organic acids derived from fast pyrolysis of lignocellulosic biomass represent a significant proportion of microbially accessible carbon in bio-oil. However, using bio-oil for microbial cultivation is a highly challenging task due to its strong adverse effects on microbial growth as well as its complex composition. In this study, the suitability of bio-oil as a substrate for industrial biotechnology was investigated with special focus on organic acids. For this purpose, using the example of the genetically engineered, non-pathogenic bacterium Pseudomonas putida KT2440 producing mono-rhamnolipids, cultivation on small organic acids derived from fast pyrolysis of lignocellulosic biomass, as well as on bio-oil fractions, was investigated and evaluated. As biosurfactants, rhamnolipids represent a potential bulk product of industrial biotechnology where substitution of traditional carbon sources is of conceivable interest. Results suggest that maximum achievable productivities as well as substrate-to-biomass yields are in a comparable range for glucose, acetate, as well as the mixture of acetate, formate and propionate. Similar yields were obtained for a pretreated bio-oil fraction, which was used as reference real raw material, although with significantly lower titers. As such, the reported process constitutes a proof-of-principle for using bio-oil as a potential cost-effective alternative carbon source in a future bio-based economy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.