ArticleProceedings of the National Academy of Sciences of the United States of America2019
Possible cooption of a VEGF-driven tubulogenesis program for biomineralization in echinoderms.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 69 citations in OpenAlex.
- Possible role of microtubules in vesicular transport of matrix protein during sea urchin larval biomineralization.Developmental dynamics : an official publication of the American Association of Anatomists · 2026Article
- Evolutionary innovation within conserved gene regulatory networks underlying biomineralized skeletons in Bilateria.Molecular biology and evolution · 2026Article
- Skeletogenic Expression of Integrin Alpha, Talin and Npnt Genes and Npnt Role in Sea Urchin Skeletogenesis.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2026Article
- Article
- Regulatory feedback between VEGF and ERK pathways controls tip-cell expression during sea urchin skeletogenesis.Development (Cambridge, England) · 2025Article
- A mechanosensitive circuit of FAK, ROCK, and ERK controls biomineral growth and morphology in the sea urchin embryo.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- ROCK and the actomyosin network control biomineral growth and morphology during sea urchin skeletogenesis.eLife · 2024Article
- microRNA-1 regulates sea urchin skeletogenesis by directly targeting skeletogenic genes and modulating components of signaling pathways.Developmental biology · 2024Article
- Sea cucumbers: an emerging system in evo-devo.EvoDevo · 2024Review
- Transcription of microRNAs is regulated by developmental signaling pathways and transcription factors.Frontiers in cell and developmental biology · 2024Article
- Cell type and gene regulatory network approaches in the evolution of spiralian biomineralisation.Briefings in functional genomics · 2023Review
- Article
- Carbonic anhydrases in development: morphological observations and gene expression profiling in sea urchin embryos exposed to acetazolamide.Open biology · 2023Article
- An optimized Tet-On system for conditional control of gene expression in sea urchins.Development (Cambridge, England) · 2023Article
- Extracellular carbonic anhydrase activity promotes a carbon concentration mechanism in metazoan calcifying cells.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Distinct regulatory states control the elongation of individual skeletal rods in the sea urchin embryo.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- Biomineralization: Integrating mechanism and evolutionary history.Science advances · 2022Review
- Cambrian edrioasteroid reveals new mechanism for secondary reduction of the skeleton in echinoderms.Proceedings. Biological sciences · 2022Article
- The Evolution of Biomineralization through the Co-Option of Organic Scaffold Forming Networks.Cells · 2022Review
- Ion Pathways in Biomineralization: Perspectives on Uptake, Transport, and Deposition of Calcium, Carbonate, and Phosphate.Journal of the American Chemical Society · 2021Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biomineralization is the process by which living organisms use minerals to form hard structures that protect and support them. Biomineralization is believed to have evolved rapidly and independently in different phyla utilizing preexisting components. The mechanistic understanding of the regulatory networks that drive biomineralization and their evolution is far from clear. Sea urchin skeletogenesis is an excellent model system for studying both gene regulation and mineral uptake and deposition. The sea urchin calcite spicules are formed within a tubular cavity generated by the skeletogenic cells controlled by vascular endothelial growth factor (VEGF) signaling. The VEGF pathway is essential for biomineralization in echinoderms, while in many other phyla, across metazoans, it controls tubulogenesis and vascularization. Despite the critical role of VEGF signaling in sea urchin spiculogenesis, the downstream program it activates was largely unknown. Here we study the cellular and molecular machinery activated by the VEGF pathway during sea urchin spiculogenesis and reveal multiple parallels to the regulation of vertebrate vascularization. Human VEGF rescues sea urchin VEGF knockdown, vesicle deposition into an internal cavity plays a significant role in both systems, and sea urchin VEGF signaling activates hundreds of genes, including biomineralization and interestingly, vascularization genes. Moreover, five upstream transcription factors and three signaling genes that drive spiculogenesis are homologous to vertebrate factors that control vascularization. Overall, our findings suggest that sea urchin spiculogenesis and vertebrate vascularization diverged from a common ancestral tubulogenesis program, broadly adapted for vascularization and specifically coopted for biomineralization in the echinoderm phylum.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.