ArticleRNA (New York, N.Y.)2019
SplintQuant: a method for accurately quantifying circular RNA transcript abundance without reverse transcription bias.
Article in RNA (New York, N.Y.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 46 citations in OpenAlex.
- Non-enzymatic conversion of RNA sequence information into DNA by squaramide ligation for accurate RNA quantification.Communications chemistry · 2026Article
- Circular RNAs in human biology: from splicing noise to master regulators.Molecular biology reports · 2026Review
- The prognostic potential of RNA in stage II colon cancer: Insights from a screened multicenter population-based cohort study.International journal of cancer · 2025Article
- Circular RNAs in Cardiovascular Physiopathology: From Molecular Mechanisms to Therapeutic Opportunities.International journal of molecular sciences · 2025Review
- Past, present, and future strategies for detecting and quantifying circular RNA variants.The FEBS journal · 2025Review
- Presence of Alzheimer's disease variants in circular RNA ofJournal of Alzheimer's disease : JAD · 2025Article
- Use of synthetic circular RNA spike-ins (SynCRS) for normalization of circular RNA sequencing data.Nature protocols · 2025Review
- Article
- Circular RNA in cancer.Nature reviews. Cancer · 2024Review
- CircRNA as an Achilles heel of cancer: characterization, biomarker and therapeutic modalities.Journal of translational medicine · 2024Review
- Long- and short-read sequencing methods discover distinct circular RNA pools in Lotus japonicus.The plant genome · 2024Article
- Single-molecule RNA sizing enables quantitative analysis of alternative transcription termination.Nature communications · 2024Article
- Multiplex Detection of RNA Viruses Based on Ligation Reaction and Universal PCR Amplification.Current microbiology · 2024Article
- Detection of viral RNAs at ambient temperature via reporter proteins produced through the target-splinted ligation of DNA probes.Nature biomedical engineering · 2023Article
- Spatial Profiling of Circular RNAs in Cancer Reveals High Expression in Muscle and Stromal Cells.Cancer research · 2023Article
- Detecting intragenic trans-splicing events from non-co-linearly spliced junctions by hybrid sequencing.Nucleic acids research · 2023Article
- Functional Characterisation of the Circular RNA,Cells · 2023Article
- Nanopore microscope identifies RNA isoforms with structural colours.Nature chemistry · 2022Article
- Best practice standards for circular RNA research.Nature methods · 2022Review
- Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reverse transcription of RNA is fallible, introducing biases and confounding the quantification of transcript abundance. We demonstrate that circular RNAs (circRNAs) are more subjective to overestimation of transcript abundance than cognate linear RNAs due to their covalently closed, circular form, producing multiple concatameric products from a single priming of reverse transcriptase. We developed SplintQuant, where custom DNA oligonucleotides are ligated by PBCV-1 DNA ligase only when bound to their target RNA. These circRNA-specific DNA oligonucleotides are terminally tagged with universal primers, allowing SplintQuant to accurately quantify even lowly abundant circRNAs through highly specific quantitative PCR (qPCR) in the absence of reverse transcription. SplintQuant is sensitive, specific, highly reproducible, and applicable to the quantification of canonical and noncanonical RNA transcripts including alternative splice variants, gene fusions, and offers a gold-standard approach for accurately quantifying circRNAs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.