Evidence map›Paper›PMID 31151204›Full record

ArticleMaterials (Basel, Switzerland)2019

Construction of 3D Cellular Composites with Stem Cells Derived from Adipose Tissue and Endothelial Cells by Use of Optical Tweezers in a Natural Polymer Solution.

Takehiro Yamazaki, Toshifumi Kishimoto, Paweł Leszczyński, Koichiro Sadakane, Takahiro Kenmotsu, Hirofumi Watanabe, Tomohiko Kazama, Taro Matsumoto, Kenichi Yoshikawa, Hiroaki Taniguchi

Open access · goldAbstract read
In one paragraph

Article in Materials (Basel, Switzerland), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Light force-powered cellular medical micromachines.Frontiers in bioengineering and biotechnology · 2026
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Takehiro YamazakiFaculty of Life and Medical Sciences, Doshisha University, Kyoto 610-0394, Japan. t.yamazaki13lifephsy@gmail.com.
Toshifumi KishimotoFaculty of Life and Medical Sciences, Doshisha University, Kyoto 610-0394, Japan. kishimotodoushisha@gmail.com.
Paweł LeszczyńskiInstitute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, 05-552 Magdalenka, Poland. p.leszczynski@ighz.pl.
Koichiro SadakaneFaculty of Life and Medical Sciences, Doshisha University, Kyoto 610-0394, Japan. sadakanekoichiro@gmail.com.
Takahiro KenmotsuFaculty of Life and Medical Sciences, Doshisha University, Kyoto 610-0394, Japan. tkenmots@mail.doshisha.ac.jp.
Hirofumi WatanabeDepartment of Functional Morphology, Division of Cell Regeneration and Transplantation, Nihon University School of Medicine, Tokyo 173-8610, Japan. watanabe.hirofumi@nihon-u.ac.jp.
Tomohiko KazamaDepartment of Functional Morphology, Division of Cell Regeneration and Transplantation, Nihon University School of Medicine, Tokyo 173-8610, Japan. kazama.tomohiko@nihon-u.ac.jp.
Taro MatsumotoDepartment of Functional Morphology, Division of Cell Regeneration and Transplantation, Nihon University School of Medicine, Tokyo 173-8610, Japan. matsumoto.taro@nihon-u.ac.jp.ORCID 0000-0002-2965-2673
Kenichi YoshikawaFaculty of Life and Medical Sciences, Doshisha University, Kyoto 610-0394, Japan. keyoshik@mail.doshisha.ac.jp.ORCID 0000-0002-2751-7136
Hiroaki TaniguchiInstitute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, 05-552 Magdalenka, Poland. h.taniguchi@ighz.pl.
Doshisha University · JPNihon University · JPPolish Academy of Sciences · PL

Funding

Japan Society for the Promotion of Science 15H02121Japan Society for the Promotion of Science 15K05400Japan Society for the Promotion of Science 50587441Krajowy Naukowy Osrodek Wiodacy No. 05-1/KNOW2/2015
6 · The paper itself

Abstract

To better understand the regulation and function of cellular interactions, three-dimensional (3D) assemblies of single cells and subsequent functional analysis are gaining popularity in many research fields. While we have developed strategies to build stable cellular structures using optical tweezers in a minimally invasive state, methods for manipulating a wide range of cell types have yet to be established. To mimic organ-like structures, the construction of 3D cellular assemblies with variety of cell types is essential. Our recent studies have shown that the presence of nonspecific soluble polymers in aqueous solution is the key to creating stable 3D cellular assemblies efficiently. The present study further expands on the construction of 3D single cell assemblies using two different cell types. We have successfully generated 3D cellular assemblies, using GFP-labeled adipose tissue-derived stem cells and endothelial cells by using optical tweezers. Our findings will support the development of future applications to further characterize cellular interactions in tissue regeneration.

Indexed as

composites with different cell typesdepletion effect of polymerendothelial cellsmesenchymal stem cellsthree-dimensional assemblies

Identifiers

PMID31151204
PMCPMC6601048
OpenAlexW2947685819

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.