ArticlePloS one2019
HPV transcription in skin tumors.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed, 12 citations in OpenAlex.
- Human papillomaviruses in hand squamous cell carcinomas from Chilean patients.Infectious agents and cancer · 2024Article
- Assessment of bacterial and viral gut communities in healthy and tumoral colorectal tissue using RNA and DNA deep sequencing.Cancer medicine · 2023Article
- Metagenomic Sequencing in the ICU for Precision Diagnosis of Critical Infectious Illnesses.Critical care (London, England) · 2023Review
- Using HPV-meta for human papillomavirus RNA quality detection.Scientific reports · 2022Article
- Comparison of DNA and RNA sequencing of total nucleic acids from human cervix for metagenomics.Scientific reports · 2021Article
- HPV infection upregulates the expression of ZNT-1 in condyloma acuminatum.European journal of histochemistry : EJH · 2021Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlthough more than 95% of viral sequences found in skin tumors typically belong to human papillomaviruses (HPVs), HPV transcription has so far not been detected. As current technology allows very deep transcriptome sequencing, we examined skin tumors and precursor lesions for HPV transcription.
methodFresh frozen biopsies from 12 skin specimens (11/12 were positive for HPV DNA) were subjected to total RNA sequencing. The cervical cancer cell line CaSki was included as a positive control for HPV transcription.
resultsHPV RNA was detected and confirmed in 1/12 skin lesions at a median depth of 66 million reads per sample. One specimen was positive for HPV 110 transcripts mapping to E6, E7, E2/E4 and L2 open reading frames, as well as to a spliced E1^E4 transcript.
conclusionIn conclusion, the study revealed that a minority of skin lesions contains HPV transcripts and that HPV DNA detection does not predict HPV transcriptional activity.
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Registered trials
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