ArticleAnalytical chemistry2019
MicroLESA: Integrating Autofluorescence Microscopy, In Situ Micro-Digestions, and Liquid Extraction Surface Analysis for High Spatial Resolution Targeted Proteomic Studies.
Article in Analytical chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- High-Throughput Analysis of Protein Adsorption to a Large Library of Polymers Using Liquid Extraction Surface Analysis-Tandem Mass Spectrometry (LESA-MS/MS).Analytical chemistry · 2025Article
- Mitochondria sense bacterial lactate and drive release of neutrophil extracellular traps.Cell host & microbe · 2025Article
- Spatial top-down proteomics for the functional characterization of human kidney.Clinical proteomics · 2025Article
- Thermal Denaturation of Fresh Frozen Tissue Enhances Mass Spectrometry Detection of Peptides.Analytical chemistry · 2024Article
- Restriction of arginine induces antibiotic tolerance in Staphylococcus aureus.Nature communications · 2024Article
- Spatial top-down proteomics for the functional characterization of human kidney.bioRxiv : the preprint server for biology · 2024Article
- Spatial Multi-Omics in Alzheimer's Disease: A Multi-Dimensional Approach to Understanding Pathology and Progression.Current issues in molecular biology · 2024Review
- Advances in Imaging Mass Spectrometry for Biomedical and Clinical Research.Trends in analytical chemistry : TRAC · 2023Article
- Proteome Mapping of the Human Pancreatic Islet Microenvironment Reveals Endocrine-Exocrine Signaling Sphere of Influence.Molecular & cellular proteomics : MCP · 2023Article
- Expanded vacuum-stable gels for multiplexed high-resolution spatial histopathology.Nature communications · 2023Article
- Rapid Multivariate Analysis Approach to Explore Differential Spatial Protein Profiles in Tissue.Journal of proteome research · 2023Article
- Nano-DESI Mass Spectrometry Imaging of Proteoforms in Biological Tissues with High Spatial Resolution.Analytical chemistry · 2023Article
- Untangling Alzheimer's disease with spatial multi-omics: a brief review.Frontiers in aging neuroscience · 2023Review
- Imaging mass spectrometry reveals complex lipid distributions acrossJournal of mass spectrometry and advances in the clinical lab · 2022Article
- Hanging drop sample preparation improves sensitivity of spatial proteomics.Lab on a chip · 2022Article
- Proteoform-Selective Imaging of Tissues Using Mass Spectrometry.Angewandte Chemie (International ed. in English) · 2022Article
- Applications of ambient ionization mass spectrometry in 2021: An annual review.Analytical science advances · 2022Review
- Uncovering Molecular Heterogeneity in the Kidney With Spatially Targeted Mass Spectrometry.Frontiers in physiology · 2022Review
- Neutrophil extracellular traps enhance macrophage killing of bacterial pathogens.Science advances · 2021Article
- Proteomic characterization of the human lens and Cataractogenesis.Expert review of proteomics · 2021Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
The ability to target discrete features within tissue using liquid surface extractions enables the identification of proteins while maintaining the spatial integrity of the sample. Here, we present a liquid extraction surface analysis (LESA) workflow, termed microLESA, that allows proteomic profiling from discrete tissue features of ∼110 μm in diameter by integrating nondestructive autofluorescence microscopy and spatially targeted liquid droplet micro-digestion. Autofluorescence microscopy provides the visualization of tissue foci without the need for chemical stains or the use of serial tissue sections. Tryptic peptides are generated from tissue foci by applying small volume droplets (∼250 pL) of enzyme onto the surface prior to LESA. The microLESA workflow reduced the diameter of the sampled area almost 5-fold compared to previous LESA approaches. Experimental parameters, such as tissue thickness, trypsin concentration, and enzyme incubation duration, were tested to maximize proteomics analysis. The microLESA workflow was applied to the study of fluorescently labeled Staphylococcus aureus infected murine kidney to identify unique proteins related to host defense and bacterial pathogenesis. Proteins related to nutritional immunity and host immune response were identified by performing microLESA at the infectious foci and surrounding abscess. These identifications were then used to annotate specific proteins observed in infected kidney tissue by MALDI FT-ICR IMS through accurate mass matching.
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