Evidence map›Paper›PMID 31149395›Full record

ArticlePeerJ2019

Purification of plant-derived anti-virus mAb through optimized pH conditions for coupling between protein A and epoxy-activated beads.

Ilchan Song, Yang Joo Kang, Su-Lim Choi, Dalmuri Han, Deuk-Su Kim, Hae Kyung Lee, Joon-Chul Lee, Jeanho Park, Do-Sun Kim, Kisung Ko

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PeerJ, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 1 country.

Ilchan Song *Department of Medicine, College of Medicine, Chung-Ang University, Seoul, South Korea.
Yang Joo Kang *Department of Medicine, College of Medicine, Chung-Ang University, Seoul, South Korea.
Su-Lim ChoiProtein Purification Laboratory, Biotech R&D Center, Amicogen, Jinju, South Korea.
Dalmuri HanDivision of Bacterial Disease Research, Korea Centers for Disease Control and Prevention, Osong, South Korea.
Deuk-Su KimDepartment of Medicine, College of Medicine, Chung-Ang University, Seoul, South Korea.
Hae Kyung LeeDivision of Bacterial Disease Research, Korea Centers for Disease Control and Prevention, Osong, South Korea.
Joon-Chul LeeResearch Institute of Industrial Technology Convergence, Korea Institute of Industrial Technology, Ansan, South Korea.
Jeanho ParkResearch Institute of Industrial Technology Convergence, Korea Institute of Industrial Technology, Ansan, South Korea.
Do-Sun KimVegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju-gun, South Korea.
Kisung KoDepartment of Medicine, College of Medicine, Chung-Ang University, Seoul, South Korea.
Chung-Ang University · KRKorea Institute of Industrial Technology · KRKorea Center for Disease Control and Prevention · KRKorea Disease Control and Prevention Agency · KRRural Development Administration · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The main goal of this research was to determine optimum pH conditions for coupling between protein A and epoxy-activated Sepharose beads for purification of monoclonal antibodies (mAbs) expressed in plants. To confirm the effect of pH conditions on purification efficacy, epoxy-activated agarose beads were coupled to protein A under the pH conditions of 8.5, 9.5, 10.5, and 11.5 (8.5R, 9.5R, 10.5R, and 11.5R, respectively). A total of 300 g of fresh leaf tissue of transgenic

Indexed as

AntibodyEpoxy-activated beadspH conditionProtein APurification

Identifiers

PMID31149395
PMCPMC6534112
OpenAlexW2945362029

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.