Evidence map›Paper›PMID 31149056›Full record

ArticleActa endocrinologica (Bucharest, Romania : 2005)

EFFECT OF DORSOMEDIAL HYPOTHALAMUS NEUROPEPTIDE Y KNOCKDOWN ON HEPATIC INSULIN SENSITIVITY.

Z Cui, Q Qin, P Chen, J Wang, S Zhang, X Mei, B Xie, S Wang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta endocrinologica (Bucharest, Romania : 2005). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 59% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Z CuiFirst Affiliated Hospital of Zhengzhou University, Department of Endocrinology and Metabolism, Zhengzhou, China.
Q QinFirst Affiliated Hospital of Zhengzhou University, Department of Endocrinology and Metabolism, Zhengzhou, China.
P ChenFirst Affiliated Hospital of Zhengzhou University, Department of Endocrinology and Metabolism, Zhengzhou, China.
J WangFirst Affiliated Hospital of Zhengzhou University, Department of Endocrinology and Metabolism, Zhengzhou, China.
S ZhangFirst Affiliated Hospital of Zhengzhou University, Department of Endocrinology and Metabolism, Zhengzhou, China.
X MeiFirst Affiliated Hospital of Zhengzhou University, Department of Endocrinology and Metabolism, Zhengzhou, China.
B XieFirst Affiliated Hospital of Zhengzhou University, Department of Endocrinology and Metabolism, Zhengzhou, China.
S WangFirst Affiliated Hospital of Zhengzhou University, Department of Endocrinology and Metabolism, Zhengzhou, China.
First Affiliated Hospital of Zhengzhou University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveIn this study we investigated the effect of dorsomedial hypothalamus (DMH) neuropeptide Y (NPY) knock-down on hepatic insulin sensitivity in high-fat (HF) diet-fed rats.

methodsForty-eight Sprague-Dawley rats were randomly assigned to receive bilateral DMH injections of adeno-associated virus AAVshNPY or AAVshCTL and then accessed to regular chow. Five weeks after viral injection, half rats in each group were given access to the HF diet. At 16 weeks, rat livers were collected. Insulin receptor substrate-1 (IRS-1) and phosphoinositide 3-kinase (PI3K) mRNA expression was measured by qRT-PCR. Blood glucose levels were measured by the oxidase method, serum insulin, triglyceride, and TC levels were measured by Elisa. Pathological changes in the liver were assessed by hematoxylin-eosin (HE) staining. AKT, p-AKT, and GSK-3 levels were measured by western blotting.

resultsCompared with AAVshCTL-injected rats, AAVshNPY-injected rats showed a significant decrease in blood glucose concentrations; serum insulin, triglyceride, and TC; HOMA-IR; and IRS-1 and PI3K mRNA levels (P<0.05). ISI, GSK-3, and p-AKT levels were significantly increased (P<0.05). HE staining showed that AAVshNPY-injected rats fed the HF diet had mild fatty degeneration.

conclusionThese results suggest that DMH NPY knock-down improves hepatic insulin sensitivity in HF diet-fed rats by activating the hepatic PI3K/AKT insulin signalling pathway.

Indexed as

Dorsomedial hypothalamusHigh fatInsulin resistanceLiver

Identifiers

PMID31149056
PMCPMC6535310
OpenAlexW2947651177

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.