Evidence map›Paper›PMID 31149024›Full record

ArticlePlant methods2019

Second generation of pepino mosaic virus vectors: improved stability in tomato and a wide range of reporter genes.

Fabiola Ruiz-Ramón, Raquel N Sempere, Eduardo Méndez-López, M Amelia Sánchez-Pina, Miguel A Aranda

Open access · goldAbstract read
In one paragraph

Article in Plant methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.0field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 23 citations in OpenAlex.

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  9. Citrus sudden death-associated virus as a new expression vector for rapidBiotechnology reports (Amsterdam, Netherlands) · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Fabiola Ruiz-RamónPresent Address: R + D+I Department, Abiopep S.L., Murcia, Spain.
Raquel N SemperePresent Address: R + D+I Department, Abiopep S.L., Murcia, Spain.
Eduardo Méndez-López2Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), Murcia, Spain.
M Amelia Sánchez-Pina2Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), Murcia, Spain.
Miguel A Aranda2Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), Murcia, Spain.ORCID 0000-0002-0828-973X
Consejo Superior de Investigaciones Científicas · ESUniversidad de Murcia · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundVectors based on plant viruses are important tools for functional genomics, cellular biology, plant genome engineering and molecular farming. We previously reported on the construction of PepGFP2a, a viral vector based on pepino mosaic virus (PepMV) which expressed GFP efficiently and stably in plants of its experimental host

resultsWe first tested PepGFPm1 and PepGFPm2, two variants of PepGFP2a in which we progressively reduced a duplication of nucleotides encoding the N-terminal region of the coat protein. The new vectors had improved GFP expression levels and stability in

conclusionsWe have significantly expanded the PepMV tool box by producing a set of new vectors with improved stability and efficiency in both

Indexed as

BARDsRedmCherryPotexvirusViral factoryViral replication complexVRC

Identifiers

PMID31149024
PMCPMC6537163
OpenAlexW2947191901

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.