ArticleScientific reports2019
Response of an actin network in vesicles under electric pulses.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Electric-field manipulation of giant vesicles: new frontiers in membrane electromechanics.European biophysics journal : EBJ · 2026Review
- Pulsed Electric Fields for Emerging Single-Cell Bioprocessing in Food Applications: Electropermeabilization Mechanisms and Design Principles.Comprehensive reviews in food science and food safety · 2026Review
- Synthesis Method and High Salt Concentration Can Affect Electrodeformation of GUVs under Strong Pulsed DC Fields.ACS omega · 2025Article
- Rearrangement of GUV-confined actin networks in response to micropipette aspiration.Cytoskeleton (Hoboken, N.J.) · 2024Article
- Thermosensory Spiking Activity of Proteinoid Microspheres Cross-Linked by Actin Filaments.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Methylation of ESCRT-III components regulates the timing of cytokinetic abscission.Nature communications · 2024Article
- Artificial cells for in vivo biomedical applications through red blood cell biomimicry.Nature communications · 2024Review
- Enhancing suspended cell transfection by inducing localized distribution of the membrane actin cortex before exposure to electromechanical stimulation.Biotechnology letters · 2023Article
- Electrochemistry in sensing of molecular interactions of proteins and their behavior in an electric field.Mikrochimica acta · 2023Review
- Differential regulation of GUV mechanics via actin network architectures.Biophysical journal · 2023Article
- Methods to mechanically perturb and characterize GUV-based minimal cell models.Computational and structural biotechnology journal · 2023Review
- Assessing membrane material properties from the response of giant unilamellar vesicles to electric fields.Advances in physics: X · 2023Article
- Article
- Studying actin-induced cell shape changes using Giant Unilamellar Vesicles and reconstituted actin networks.Biochemical Society transactions · 2022Review
- Modeling the gene delivery process of the needle array-based tissue nanotransfection.Nano research · 2022Article
- Identification of electroporation sites in the complex lipid organization of the plasma membrane.eLife · 2022Article
- Mechanical Stimulation after Centrifuge-Free Nano-Electroporative Transfection Is Efficient and Maintains Long-Term T Cell Functionalities.Small (Weinheim an der Bergstrasse, Germany) · 2021Article
- To Close or to Collapse: The Role of Charges on Membrane Stability upon Pore Formation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Pulsed Electric Fields Promote Liposome Buddings.Bioelectricity · 2021Article
- Single Cell Forces after Electroporation.ACS nano · 2021Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We study the role of a biomimetic actin network during the application of electric pulses that induce electroporation or electropermeabilization, using giant unilamellar vesicles (GUVs) as a model system. The actin cortex, a subjacently attached interconnected network of actin filaments, regulates the shape and mechanical properties of the plasma membrane of mammalian cells, and is a major factor influencing the mechanical response of the cell to external physical cues. We demonstrate that the presence of an actin shell inhibits the formation of macropores in the electroporated GUVs. Additionally, experiments on the uptake of dye molecules after electroporation show that the actin network slows down the resealing process of the permeabilized membrane. We further analyze the stability of the actin network inside the GUVs exposed to high electric pulses. We find disruption of the actin layer that is likely due to the electrophoretic forces acting on the actin filaments during the permeabilization of the GUVs. Our findings on the GUVs containing a biomimetic network provide a step towards understanding the discrepancies between the electroporation mechanism of a living cell and its simplified model of the empty GUV.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.