ArticleJournal of biomedical optics2019
Second-harmonic generation microscopy analysis reveals proteoglycan decorin is necessary for proper collagen organization in prostate.
Article in Journal of biomedical optics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
- Generalized 3D quasi-phase-matching model of image contrast in second harmonic generation microscopy of fibrillar collagen architectures.JPhys photonics · 2026Article
- Prostate Cancer-Associated Fibroblasts: A Review on CAF Functions, Heterogeneity, Resistance Mechanisms, and Future in a Chip.International journal of molecular sciences · 2026Review
- Generation of a novel lung fibrosis model using precision-cut lung slices from transgenic TGFβ1 mice.American journal of physiology. Cell physiology · 2025Article
- Mechanisms on How Matricellular Microenvironments Sustain Idiopathic Pulmonary Fibrosis.International journal of molecular sciences · 2025Review
- Customized Heparinized Alginate and Collagen Hydrogels for Tunable, Local Delivery of Angiogenic Proteins.ACS biomaterials science & engineering · 2025Article
- Nonlinear microscopy and deep learning classification for mammary gland microenvironment studies.Biomedical optics express · 2023Article
- Stromal architecture directs early dissemination in pancreatic ductal adenocarcinoma.JCI insight · 2022Article
- Assessing collagen alterations in enzymatic degradation models of osteoarthritis via second harmonic generation microscopy.Osteoarthritis and cartilage · 2021Article
- Aligned forces: Origins and mechanisms of cancer dissemination guided by extracellular matrix architecture.Current opinion in cell biology · 2021Review
- Recent Advancements in Optical Harmonic Generation Microscopy: Applications and Perspectives.BME frontiers · 2021Review
- Article
- Second-harmonic generation directionality is associated with neoadjuvant chemotherapy response in breast cancer core needle biopsies.Journal of biomedical optics · 2019Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Collagen remodeling occurs in many prostate pathologies; however, the underlying structural architecture in both normal and diseased prostatic tissues is largely unexplored. Here, we use second-harmonic generation (SHG) microscopy to specifically probe the role of the proteoglycan decorin (Dcn) on collagen assembly in a wild type (wt) and Dcn null mouse (Dcn - / - ). Dcn is required for proper organization of collagen fibrils as it regulates size by forming an arch-like structure at the end of the fibril. We have utilized SHG metrics based on emission directionality (forward-backward ratio) and relative conversion efficiency, which are both related to the SHG coherence length, and found more disordered fibril organization in the Dcn - / - . We have also used image analysis readouts based on entropy, multifractal dimension, and wavelet transforms to compare the collagen fibril/fiber architecture in the two models, where all these showed that the Dcn - / - prostate comprised smaller and more disorganized collagen structures. All these SHG metrics are consistent with decreased SHG phase matching in the Dcn - / - and are further consistent with ultrastructural analysis of collagen in this model in other tissues, which show a more random distribution of fibril sizes and their packing into fibers. As Dcn is a known tumor suppressor, this work forms the basis for future studies of collagen remodeling in both malignant and benign prostate disease.
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Registered trials
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