Evidence map›Paper›PMID 31147908›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2019

Conditional Mutation of SMC5 in Mouse Embryonic Fibroblasts.

Himaja Gaddipati, Marina V Pryzhkova, Philip W Jordan

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

  1. International journal of molecular sciences · 2023
    Article
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Himaja GaddipatiDepartment of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA.
Marina V PryzhkovaDepartment of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA.
Philip W JordanDepartment of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA. pjordan8@jhu.edu.
Johns Hopkins University · US

Funding

Regulation of microtubule organizing centers during mammalian gametogenesisR01GM117155 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI Philip W Jordan · 2016 to 2026
$4.3M
The SMC5/6 Complex - DNA Damage Response Regulation Ensures Meiotic FidelityR00HD069458 · NICHD · JOHNS HOPKINS UNIVERSITY · PI JORDAN, PHILIP W · 2013 to 2015
$613k
The SMC5/6 Complex - DNA Damage Response Regulation Ensures Meiotic FidelityK99HD069458 · NICHD · JACKSON LABORATORY · PI JORDAN, PHILIP W · 2011 to 2012
$180k
NICHD NIH HHS K99 HD069458NICHD NIH HHS R00 HD069458NIGMS NIH HHS R01 GM117155
6 · The paper itself

Abstract

The structural maintenance of chromosomes (SMC) complex, SMC5/6, is important for genome maintenance in all model eukaryotes. To date, the most extensive studies have focused on the roles of Smc5/6 in lower eukaryotes, such as yeast and fly. In the handful of studies that have used mammalian cells, siRNA was used by most to knockdown SMC5/6 components. RNAi methods have been very important for scientific progression, but they are hindered by incomplete silencing of protein expression and off-target effects. This chapter outlines the use of a conditional knockout approach in mouse embryonic fibroblasts to study the function of the SMC5/6 complex. These cell lines provide an alternative method to study the function and properties of the SMC5/6 complex in mammals.

Indexed as

AnimalsCell Cycle ProteinsCell LineChromosomesFibroblastsGenomeMiceMice, KnockoutMutationNIH 3T3 CellsRNA InterferenceCell Cycle ProteinsSMC5 protein, mouseChromosome segregationConditional knockoutDNA bridgesDNA damageMicronucleiMitotic catastropheMouse embryonic fibroblastSMC5/6Structural maintenance of chromosomes

Identifiers

PMID31147908
PMCPMC6853063
OpenAlexW2947337865

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.