ArticleJournal of the American College of Cardiology2019
Modeling Cardiovascular Risks of E-Cigarettes With Human-Induced Pluripotent Stem Cell-Derived Endothelial Cells.
Article in Journal of the American College of Cardiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
87 citing papers in PubMed.
- Impact of Heated Tobacco Products, E-Cigarettes, and Cigarettes on Inflammation and Endothelial Dysfunction.International journal of molecular sciences · 2023Trial
- High-Throughput Digital Decoding of Vascular Heterogeneity in Patient-Specific Tumor Microenvironments.Advanced healthcare materials · 2026Article
- Nicotine-Mediated Redox and Nitrosative Stress in Cardiorenal Health.Hypertension (Dallas, Tex. : 1979) · 2026Review
- Human Primary Mesenchymal Stem Cells Fail to Recover Full Osteogenic Differentiation Potential Following Washout of Electronic Cigarette Aerosol Extracts.Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco · 2026Article
- Roles of Catecholamines and Inflammation in Endothelial Dysfunction: a Study Using Takotsubo Syndrome Patient-specific hiPSC-ECs.Stem cell reviews and reports · 2026Article
- Cardiovascular Consequences of E-Cigarettes: A New Challenge for Cardiologists.Journal of clinical medicine · 2026Review
- Molecular Health Effects of Electronic Cigarettes.Biomolecules · 2026Review
- New-onset atrial fibrillation in a healthy young adult following electronic cigarette use.Journal of Taibah University Medical Sciences · 2026Article
- E-cigarettes and cardiovascular health: a review of components, mechanisms, and clinical risk.American journal of cardiovascular disease · 2026Review
- Concurrent e-cigarette or vaping product use-associated lung injury (EVALI) and myopericarditis: a rare case report and literature review.BMC pulmonary medicine · 2025Review
- Impact of Nicotine-Free Electronic Cigarettes on Cardiovascular Health: A Systematic Review.Journal of clinical medicine · 2025Review
- Exploring the Impact of E-cigarettes on Cardiovascular Health: Insights from Preclinical and Clinical Studies.Cardiovascular toxicology · 2025Review
- Review
- Polarization of THP-1-Derived Human M0 to M1 Macrophages Exposed to Flavored E-Liquids.Toxics · 2025Article
- Disposable e-cigarettes and their nicotine delivery, usage pattern, and subjective effects in occasionally smoking adults.Scientific reports · 2025Article
- The impact of smoked cigarettes' type on the level of reactive oxygen species in physicians with surgical and non-surgical specialization.BMC pulmonary medicine · 2025Article
- Pathophysiological Responses of Oral Keratinocytes After Exposure to Flavored E-Cigarette Liquids.Dentistry journal · 2025Article
- Oxidative balance score and menopausal status: insights from epidemiological analysis and machine learning models.Frontiers in nutrition · 2025Article
- Advancing Environmental ToxicologyEnvironment & health (Washington, D.C.) · 2024Review
- Cigarettes and e-cigarettes use among US adults with multimorbidity.Drug and alcohol dependence reports · 2024Article
27 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
14 authors.
Funding
Abstract
backgroundElectronic cigarettes (e-cigarettes) have experienced a tremendous increase in use. Unlike cigarette smoking, the effects of e-cigarettes and their constituents on mediating vascular health remain understudied. However, given their increasing popularity, it is imperative to evaluate the health risks of e-cigarettes, including the effects of their ingredients, especially nicotine and flavorings.
objectivesThe purpose of this study was to investigate the effects of flavored e-cigarette liquids (e-liquids) and serum isolated from e-cigarette users on endothelial health and endothelial cell-dependent macrophage activation.
methodsHuman-induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) and a high-throughput screening approach were used to assess endothelial integrity following exposure to 6 different e-liquids with varying nicotine concentrations and to serum from e-cigarette users.
resultsThe cytotoxicity of the e-liquids varied considerably, with the cinnamon-flavored product being most potent and leading to significantly decreased cell viability, increased reactive oxygen species (ROS) levels, caspase 3/7 activity, and low-density lipoprotein uptake, activation of oxidative stress-related pathway, and impaired tube formation and migration, confirming endothelial dysfunction. Upon exposure of ECs to e-liquid, conditioned media induced macrophage polarization into a pro-inflammatory state, eliciting the production of interleukin-1β and -6, leading to increased ROS. After exposure of human iPSC-ECs to serum of e-cigarette users, increased ROS linked to endothelial dysfunction was observed, as indicated by impaired pro-angiogenic properties. There was also an observed increase in inflammatory cytokine expression in the serum of e-cigarette users.
conclusionsAcute exposure to flavored e-liquids or e-cigarette use exacerbates endothelial dysfunction, which often precedes cardiovascular diseases.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.