ArticlePLoS neglected tropical diseases2019
Whole genome sequence of Vibrio cholerae directly from dried spotted filter paper.
Article in PLoS neglected tropical diseases, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 15 citations in OpenAlex.
- Multicountry genomic analysis underscores regional cholera spread in Africa.Nature communications · 2026Article
- Identification, Comparison, and Profiling of Selected Diarrhoeagenic Pathogens from Diverse Water Sources and Human and Animal Faeces Using Whole-Genome Sequencing.Microorganisms · 2025Article
- Whole genome sequencing and transmission analysis ofmedRxiv : the preprint server for health sciences · 2025Article
- Growth response ofIranian journal of microbiology · 2024Article
- Vibrio-Sequins - dPCR-traceable DNA standards for quantitative genomics of Vibrio spp.BMC genomics · 2023Article
- Putting rapid tests to work in surveillance and control of cholera.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2022Article
- Cholera rapid diagnostic tests recycled for PCR confirmation.The Lancet. Global health · 2022Article
- Spread and Endemicity of Cholera in India: Factors Beyond the Numbers.The Journal of infectious diseases · 2021Article
- Three transmission events of Vibrio cholerae O1 into Lusaka, Zambia.BMC infectious diseases · 2021Article
- On the transformation of MinHash-based uncorrected distances into proper evolutionary distances for phylogenetic inference.F1000Research · 2020Article
Corrections and comments
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Authors and funding
13 authors at 7 institutions in 5 countries.
Funding
Abstract
backgroundGlobal estimates for cholera annually approximate 4 million cases worldwide with 95,000 deaths. Recent outbreaks, including Haiti and Yemen, are reminders that cholera is still a global health concern. Cholera outbreaks can rapidly induce high death tolls by overwhelming the capacity of health facilities, especially in remote areas or areas of civil unrest. Recent studies demonstrated that stool specimens preserved on filter paper facilitate molecular analysis of Vibrio cholerae in resource limited settings. Specimens preserved in a rapid, low-cost, safe and sustainable manner for sequencing provides previously unavailable data about circulating cholera strains. This may ultimately contribute new information to shape public policy response on cholera control and elimination. METHODOLOGY/PRINCIPAL
findingsWhole genome sequencing (WGS) recovered close to a complete sequence of the V. cholerae O1 genome with satisfactory genome coverage from stool specimens enriched in alkaline peptone water (APW) and V. cholerae culture isolates, both spotted on filter paper. The minimum concentration of V. cholerae DNA sufficient to produce quality genomic information was 0.02 ng/μL. The genomic data confirmed the presence or absence of genes of epidemiological interest, including cholera toxin and pilus loci. WGS identified a variety of diarrheal pathogens from APW-enriched specimen spotted filter paper, highlighting the potential for this technique to explore the gut microbiome, potentially identifying co-infections, which may impact the severity of disease. WGS demonstrated that these specimens fit within the current global cholera phylogenetic tree, identifying the strains as the 7th pandemic El Tor.
conclusionsWGS results allowed for mapping of short reads from APW-enriched specimen and culture isolate spotted filter papers. This provided valuable molecular epidemiological sequence information on V. cholerae strains from remote, low-resource settings. These results identified the presence of co-infecting pathogens while providing rare insight into the specific V. cholerae strains causing outbreaks in cholera-endemic areas.
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