ArticlePhysical review letters2019
Fundamental Limits on Measuring the Rotational Constraint of Single Molecules Using Fluorescence Microscopy.
Article in Physical review letters, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Optimal excitation polarization schemes to boost the precision of single-molecule orientation imaging.Biomedical optics express · 2026Article
- Single Fluorogens and Orientation-Localization Microscopy for Quantifying Chemical and Biomolecular Dynamics at the Nanoscale.Accounts of chemical research · 2026Article
- Single molecule orientation and localization microscopy.Nature photonics · 2025Article
- Resolving the Orientations of and Angular Separation Between a Pair of Dipole Emitters.Physical review letters · 2025Article
- Single-molecule orientation-localization microscopy: Applications and approaches.Quarterly reviews of biophysics · 2024Review
- Fundamental Limits in Measuring the Anisotropic Rotational Diffusion of Single Molecules.The journal of physical chemistry. A · 2024Article
- Deep-SMOLM: deep learning resolves the 3D orientations and 2D positions of overlapping single molecules with optimal nanoscale resolution.Optics express · 2022Article
- Article
- Resolving the Three-Dimensional Rotational and Translational Dynamics of Single Molecules Using Radially and Azimuthally Polarized Fluorescence.Nano letters · 2022Article
- 4polar-STORM polarized super-resolution imaging of actin filament organization in cells.Nature communications · 2022Article
- Single-Molecule Localization Microscopy of 3D Orientation and Anisotropic Wobble Using a Polarized Vortex Point Spread Function.The journal of physical chemistry. B · 2021Article
- Simultaneous orientation and 3D localization microscopy with a Vortex point spread function.Nature communications · 2021Article
- Elucidating the Role of Topological Constraint on the Structure of Overstretched DNA Using Fluorescence Polarization Microscopy.The journal of physical chemistry. B · 2021Article
- Birefringent Fourier filtering for single molecule coordinate and height super-resolution imaging with dithering and orientation.Nature communications · 2020Article
- Variable incidence angle linear dichroism (VALiD): a technique for unique 3D orientation measurement of fluorescent ensembles.Optics express · 2020Article
- Spatio-angular fluorescence microscopy II. Paraxial 4f imaging.Journal of the Optical Society of America. A, Optics, image science, and vision · 2019Article
- Spatio-angular fluorescence microscopy I. Basic theory.Journal of the Optical Society of America. A, Optics, image science, and vision · 2019Article
- Quantum limits for precisely estimating the orientation and wobble of dipole emitters.Physical review researchArticle
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Optical fluorescence imaging is capable of measuring both the translational and rotational dynamics of single molecules. However, unavoidable measurement noise will result in inaccurate estimates of rotational dynamics, causing a molecule to appear to be more rotationally constrained than it actually is. We report a mathematical framework to compute the fundamental limit of accuracy in measuring the rotational mobility of dipolelike emitters. By applying our framework to both in-plane and three-dimensional methods, we provide a means to choose the optimal orientation-measurement technique based on experimental conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.