ArticleMetabolomics : Official journal of the Metabolomic Society2019
Integrated metabolomics identifies CYP72A67 and CYP72A68 oxidases in the biosynthesis of Medicago truncatula oleanate sapogenins.
Article in Metabolomics : Official journal of the Metabolomic Society, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 44 citations in OpenAlex.
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- Integrating metabolomic and transcriptomic analyses to identify candidate genes and transcriptional regulators associated with triterpenoid saponin accumulation in developingFrontiers in plant science · 2026Article
- Engineering Gypsophila elegans hairy root cultures to produce endosomal escape-enhancing saponins.Plant biotechnology journal · 2025Article
- Telomere-to-telomere gap-free genome assembly provides genetic insight into the triterpenoid saponins biosynthesis inHorticulture research · 2025Article
- Identification and Expression Analysis of Cytochrome P450 Genes Probably Involved in Triterpenoid Saponins Biosynthesis inInternational journal of molecular sciences · 2024Article
- Hydroxylases involved in terpenoid biosynthesis: a review.Bioresources and bioprocessing · 2023Review
- Full-length transcriptome, proteomics and metabolite analysis reveal candidate genes involved triterpenoid saponin biosynthesis inFrontiers in plant science · 2023Article
- Switchgrass Metabolomics Reveals Striking Genotypic and Developmental Differences in Specialized Metabolic Phenotypes.Journal of agricultural and food chemistry · 2022Article
- Cytochrome P450 monooxygenase-mediated tailoring of triterpenoids and steroids in plants.Beilstein journal of organic chemistry · 2022Review
- Cytochrome P450 Monooxygenase/Cytochrome P450 Reductase Bi-Enzymatic System Isolated FromFrontiers in plant science · 2022Article
- Genome-Wide Identification and Analyses of Drought/Salt-Responsive CytochromeInternational journal of molecular sciences · 2021Article
- Biological Activities and Chemistry of Triterpene Saponins fromEvidence-based complementary and alternative medicine : eCAM · 2021Article
- CRISPR/Cas9-Mediated Targeted Mutagenesis ofFrontiers in plant science · 2021Article
- Full-Length Transcriptome Analyses of Genes Involved in Triterpenoid Saponin Biosynthesis ofFrontiers in genetics · 2021Article
- A Seed-Specific Regulator of Triterpene Saponin Biosynthesis inThe Plant cell · 2020Article
- Large-Scale Profiling of Saponins in Different Ecotypes ofFrontiers in plant science · 2019Article
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12 authors at 5 institutions in 5 countries.
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No grant is acknowledged in the PubMed record.
Abstract
introductionTriterpene saponins are important bioactive plant natural products found in many plant families including the Leguminosae.
objectivesWe characterize two Medicago truncatula cytochrome P450 enzymes, MtCYP72A67 and MtCYP72A68, involved in saponin biosynthesis including both in vitro and in planta evidence.
methodsUHPLC-(-)ESI-QToF-MS was used to profile saponin accumulation across a collection of 106 M. truncatula ecotypes. The profiling results identified numerous ecotypes with high and low saponin accumulation in root and aerial tissues. Four ecotypes with significant differential saponin content in the root and/or aerial tissues were selected, and correlated gene expression profiling was performed.
resultsCorrelation analyses between gene expression and saponin accumulation revealed high correlations between saponin content with gene expression of β-amyrin synthase, MtCYP716A12, and two cytochromes P450 genes, MtCYP72A67 and MtCYP72A68. In vivo and in vitro biochemical assays using yeast microsomes containing MtCYP72A67 revealed hydroxylase activity for carbon 2 of oleanolic acid and hederagenin. This finding was supported by functional characterization of MtCYP72A67 using RNAi-mediated gene silencing in M. truncatula hairy roots, which revealed a significant reduction of 2β-hydroxylated sapogenins. In vivo and in vitro assays with MtCYP72A68 produced in yeast showed multifunctional oxidase activity for carbon 23 of oleanolic acid and hederagenin. These findings were supported by overexpression of MtCYP72A68 in M. truncatula hairy roots, which revealed significant increases of oleanolic acid, 2β-hydroxyoleanolic acid, hederagenin and total saponin levels.
conclusionsThe cumulative data support that MtCYP72A68 is a multisubstrate, multifunctional oxidase and MtCYP72A67 is a 2β-hydroxylase, both of which function during the early steps of triterpene-oleanate sapogenin biosynthesis.
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