ArticleBiomedical optics express2019
Elastography of multicellular spheroids using 3D light microscopy.
Article in Biomedical optics express, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Sequential orthogonal assays for longitudinal and endpoint characterization of three-dimensional spheroids.Nature protocols · 2025Review
- Origin of yield stress and mechanical plasticity in model biological tissues.Nature communications · 2025Article
- Lightsheet microscopy integrates single-cell optical visco-elastography and fluorescence cytometry of 3D live tissues.bioRxiv : the preprint server for biology · 2024Article
- Multiscale elasticity mapping of biological samples in 3D at optical resolution.Acta biomaterialia · 2024Article
- Optical Elastography for Micropressure Characterization of Zebrafish Embryonic Cardiac Development.Annals of biomedical engineering · 2024Article
- Label-Free Morphological Phenotyping of In Vitro 3D Microtumors.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Nuclear envelope mechanobiology: linking the nuclear structure and function.Nucleus (Austin, Tex.) · 2021Review
- Mechanical properties of cell sheets and spheroids: the link between single cells and complex tissues.Biophysical reviews · 2021Review
- Image-Based Elastography of Heterochromatin and Euchromatin Domains in the Deforming Cell Nucleus.Small (Weinheim an der Bergstrasse, Germany) · 2021Article
- Studies of nanoparticle delivery withBioactive materials · 2020Review
- The Actuation Mechanism of 3D Printed Flexure-Based Robotic Microtweezers.Micromachines · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have demonstrated a new method of 3D elastography based on 3D light microscopy and micro-scale manipulation. We used custom-built micromanipulators to apply a mechanical force onto multicellular tumor spheroids (200-300 µm in size) and recorded the induced compression with a differential interference contrast (DIC)/confocal microscope to obtain a 4D (x, y, z, and indentation steps) image sequence. Deformation analysis made through 3D pattern tracking without using fluorescence revealed 3D structural and spatial heterogeneity in tumor spheroids. We observed a 20-30 µm-sized spot of locally-induced large deformation within a tumor spheroid. We also found solid fibroblast cores formed in a tumor-fibroblast co-culture spheroid to be stiffer than surrounding cancer cells, which would not have been discovered using only conventional fluorescence. Our new method of 3D elastography may be used to better understand structural composition in multicellular spheroids through analysis of mechanical heterogeneity.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.