ArticleBiotechnology for biofuels2019
A screening method to identify efficient sgRNAs in Arabidopsis, used in conjunction with cell-specific lignin reduction.
Article in Biotechnology for biofuels, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Evaluation of computational tools for the prediction of CRISPR/SpCas9 gRNA activity in plants.Plant cell reports · 2026Article
- Article
- Harnessing promoter elements to enhance gene editing in plants: perspectives and advances.Plant biotechnology journal · 2025Review
- Advances in lignocellulosic feedstocks for bioenergy and bioproducts.Nature communications · 2025Review
- Putative rhamnogalacturonan-II glycosyltransferase identified through callus gene editing which bypasses embryo lethality.Plant physiology · 2024Article
- Application of multiple sgRNAs boosts efficiency of CRISPR/Cas9-mediated gene targeting in Arabidopsis.BMC biology · 2024Article
- Breeding and biotechnology approaches to enhance the nutritional quality of rapeseed byproducts for sustainable alternative protein sources- a critical review.Frontiers in plant science · 2024Review
- Knockout of floral and meiosis genes using CRISPR/Cas9 produces male-sterility in Eucalyptus without impacts on vegetative growth.Plant direct · 2023Article
- CRISPR/Cas9 Based Cell-Type Specific Gene Knock-Out inPlants (Basel, Switzerland) · 2023Article
- Seedlessness Trait and Genome Editing-A Review.International journal of molecular sciences · 2023Review
- Evaluate the guide RNA effectivenessFrontiers in plant science · 2023Article
- Conditional and tissue-specific approaches to dissect essential mechanisms in plant development.Current opinion in plant biology · 2022Review
- Progress and challenges in sorghum biotechnology, a multipurpose feedstock for the bioeconomy.Journal of experimental botany · 2022Review
- Spatio-Temporal Modification of Lignin Biosynthesis in Plants: A Promising Strategy for Lignocellulose Improvement and Lignin Valorization.Frontiers in bioengineering and biotechnology · 2022Review
- Spatiotemporal Regulation of CRISPR/Cas9 Enables Efficient, Precise, and Heritable Edits in Plant Genomes.Frontiers in genome editing · 2022Review
- Harnessing tissue-specific genome editing in plants through CRISPR/Cas system: current state and future prospects.Planta · 2021Review
- A gene-editing/complementation strategy for tissue-specific lignin reduction while preserving biomass yield.Biotechnology for biofuels · 2021Article
- Advances in application of genome editing in tomato and recent development of genome editing technology.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Review
- Lateral Root Initiation and the Analysis of Gene Function Using Genome Editing with CRISPR inGenes · 2021Review
- Targeted plant improvement through genome editing: from laboratory to field.Plant cell reports · 2021Review
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSingle guide RNA (sgRNA) selection is important for the efficiency of CRISPR/Cas9-mediated genome editing. However, in plants, the rules governing selection are not well established.
resultsWe developed a facile transient assay to screen sgRNA efficiency. We then used it to test top-performing bioinformatically predicted sgRNAs for two different Arabidopsis genes. In our assay, these sgRNAs had vastly different editing efficiencies, and these efficiencies were replicated in stably transformed Arabidopsis lines. One of the genes,
conclusionsThis screening method is widely applicable, and the selection and use of efficient sgRNAs will accelerate the process of expanding germplasm for both molecular breeding and research. In addition, this, to the best of our knowledge, is the first application of constrained genome editing to obtain chimeric plants of essential genes, thereby providing a dominant method to avoid lethal growth phenotypes.
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