ArticleScientific reports2019
Engineering the vasculature of decellularized rat kidney scaffolds using human induced pluripotent stem cell-derived endothelial cells.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 61 citations in OpenAlex.
- Current strategies on kidney regeneration using tissue engineering approaches: a systematic review.BMC nephrology · 2025Pooled it
- A Radiopaque and Thermoresponsive Nanogel-Based Pickering Emulsion for Permanent Arterial Embolization.ACS omega · 2026Article
- Strategies for the vascular patterning of engineered tissues for organ repair.Nature biomedical engineering · 2025Review
- A human stem cell-derived model reveals pathologic extracellular matrix remodeling in diabetic podocyte injury.Matrix biology plus · 2024Article
- Fenestrated Endothelial Cells across Organs: Insights into Kidney Function and Disease.International journal of molecular sciences · 2024Review
- Decellularized extracellular matrix biomaterials for regenerative therapies: Advances, challenges and clinical prospects.Bioactive materials · 2024Review
- Vascularization of kidney organoids: different strategies and perspectives.Frontiers in urology · 2024Review
- Current status and future prospects of decellularized kidney tissue.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2023Review
- Hypoimmunogenic Human Pluripotent Stem Cells as a Powerful Tool for Liver Regenerative Medicine.International journal of molecular sciences · 2023Article
- Decellularized Extracellular Matrix: The Role of This Complex Biomaterial in Regeneration.ACS omega · 2023Review
- Effect of different decellularization protocols on reendothelialization with human cells for a perfused renal bioscaffold of the rat.BMC biotechnology · 2023Article
- Still finding ways to augment the existing management of acute and chronic kidney diseases with targeted gene and cell therapies: Opportunities and hurdles.Frontiers in medicine · 2023Review
- Pushing the boundaries of innovation: the potential ofFrontiers in cardiovascular medicine · 2023Review
- A Combination of Physical and Chemical Treatments Is More Effective in The Preparation of Acellular Uterine Scaffolds.Cell journal · 2023Article
- Angiogenesis and Re-endothelialization in decellularized scaffolds: Recent advances and current challenges in tissue engineering.Frontiers in bioengineering and biotechnology · 2023Review
- Vascular reconstruction of the decellularized biomatrix for whole-organ engineering-a critical perspective and future strategies.Frontiers in bioengineering and biotechnology · 2023Review
- Effect of Co-culture of mesenchymal stem cell and glomerulus endothelial cell to promote endothelialization under optimized perfusion flow rate in whole renal ECM scaffold.Materials today. Bio · 2022Article
- Generation of chimeric kidneys using progenitor cell replacement: Oshima Award Address 2021.Clinical and experimental nephrology · 2022Review
- Flow-induced glycocalyx formation and cell alignment of HUVECs compared to iPSC-derived ECs for tissue engineering applications.Frontiers in cell and developmental biology · 2022Article
- Potential Strategies for Kidney Regeneration With Stem Cells: An Overview.Frontiers in cell and developmental biology · 2022Review
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Generating new kidneys using tissue engineering technologies is an innovative strategy for overcoming the shortage of donor organs for transplantation. Here we report how to efficiently engineer the kidney vasculature of decellularized rat kidney scaffolds by using human induced pluripotent stem cell (hiPSCs)-derived endothelial cells (hiPSC-ECs). In vitro, hiPSC-ECs responded to flow stress by acquiring an alignment orientation, and attached to and proliferated on the acellular kidney sections, maintaining their phenotype. The hiPSC-ECs were able to self-organize into chimeric kidney organoids to form vessel-like structures. Ex vivo infusion of hiPSC-ECs through the renal artery and vein of acellular kidneys resulted in the uniform distribution of the cells in all the vasculature compartments, from glomerular capillaries to peritubular capillaries and small vessels. Ultrastructural analysis of repopulated scaffolds through transmission and scanning electron microscopy demonstrated the presence of continuously distributed cells along the vessel wall, which was also confirmed by 3D reconstruction of z-stack images showing the continuity of endothelial cell coverage inside the vessels. Notably, the detection of fenestrae in the endothelium of glomerular capillaries but not in the vascular capillaries was clear evidence of site-specific endothelial cell specialisation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.